CAJ | 학술논문

目的分析海马细胞外氨基酸递质在癫痫发生中的作用,探讨海人酸致痫模型大鼠癫痫发生的机制.方法应用立体定向方法建立海人酸大鼠颞叶癫痫模型,观察大鼠行为学和电生理变化,应用电镜观察大鼠海马超微结构,应用微透析获取大鼠海马细胞外液,高压液相色谱法测定透析液中的兴奋性氨基酸谷氨酸、抑制性氨基酸牛磺酸及γ-氨基丁酸的含量.结果海人酸注射后大鼠出现典型的颞叶癫痫发作,皮层脑电显示痫性发作,电镜显示兴奋性神经递质增加,高效液相色谱分析显示海马细胞外谷氨酸、牛磺酸和γ-氨基丁酸含量明显高于对照组(P＜0.05),虽然谷氨酸、γ-氨基丁酸都升高,而谷氨酸升高的更明显.结论兴奋性氨基酸与抑制性氨基酸的失衡在海人酸致痫大鼠模型的癫痫发生过程中发挥重要作用,是癫痫发生的原因之一.
목적 분석해마세포외안기산체질재전간발생중적작용,탐토해인산치간모형대서전간발생적궤제.방법 응용입체정향방법건립해인산대서섭협전간모형,관찰대서행위학화전생리변화,응용전경관찰대서해마초미결구,응용미투석획취대서해마세포외액,고압액상색보법측정투석액중적흥강성안기산곡안산、억제성안기산우광산급γ-안기정산적함량.결과 해인산주사후대서출현전형적섭협전간발작,피층뇌전현시간성발작,전경현시흥강성신경체질증가,고효액상색보분석현시해마세포외곡안산、우광산화γ-안기정산함량명현고우대조조(P＜0.05),수연곡안산、γ-안기정산도승고,이곡안산승고적경명현.결론 흥강성안기산여억제성안기산적실형재해인산치간대서모형적전간발생과정중발휘중요작용,시전간발생적원인지일.
Objective To establish and evaluate the animal model for temporal lobe epilepsy kindled by kainic acid (KA)in rats,to observe the changes in glutamic amino acid (Glu),taurine amino acid (Tau),and γ-aminobatyric amino acid (GABA) of the extracellular fluid in the hippocampus of kindled rats,and to analyze the role of neurotransmitters in epileptogenesis.Methods In order to establish an animal model for temporal lobe epilepsy in rats,the characteristics of praxiological changes,electrophysiological changes,and ultrastructural examinations were observed.The contents of Glu,Tau,and GABA were detected in the hypppocampus by microdialysis combined with high performance liquid chromatography (HPLC).Results Typical seizures were observed in the models.Epileptic discharges,such as spike,slow,spike-slow,and sharp waves appeared in the electroencephalogram (EEG).Electron microscopic analysis of the hippocampal tissues revealed an increase of excitatory amino acid levels.The levels of Glu,Tau,and GABA were higher than groups that were injected with normal sodium (P ＜ 0.05).Conclusion Excitatory amino acids and inhibitory amino acids in the presence of epilepsy play important roles and is one of the main causes of seizure.