中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2013年
1期
85-89
,共5页
陆士奇%孙海伟%刘励军%张洪高%郭凌川%惠国桢
陸士奇%孫海偉%劉勵軍%張洪高%郭凌川%惠國楨
륙사기%손해위%류려군%장홍고%곽릉천%혜국정
脊髓损伤%脐带间充质干细胞%骨髓间充质干细胞%免疫排斥反应%功能修复
脊髓損傷%臍帶間充質榦細胞%骨髓間充質榦細胞%免疫排斥反應%功能脩複
척수손상%제대간충질간세포%골수간충질간세포%면역배척반응%공능수복
Spinal cord injury%Umbilical cord mesenchymal stem cells%Bone marrow mesenchymal stem cells%Immunological rejection%Functional recovery
目的 观察人脐带间充质干细胞(HUCMSCs)与大鼠骨髓间充质干细胞(BMSCs)移植治疗大鼠脊髓损伤后的功能恢复和急慢性排斥反应.方法 选取SD大鼠40只按随机数字表随机分成3组:脐带间充质干细胞移植组16只、骨髓间充质干细胞移植组16只和PBS组8只.采用脊髓半切手术制作大鼠脊髓损伤模型,损伤后对脐带间充质干细胞移植组进行HUCMSCs移植,对骨髓间充质干细胞移植组行大鼠BMSCs移植,对PBS组移植同等剂量的PBS.通过BBB评分对各组动物的术后恢复进行评估比较.用ELISA法测定移植后3组动物不同时间点(移植后1周、1个月、2个月及3个月)血中IL-2及IL-10的水平,并通过免疫组化的方法检测移植局部病理切片CD3+细胞浸润的情况.结果 在移植后1周、1个月、2个月及3个月时,检测到干细胞移植组各个时间点大鼠静脉血中IL-2水平较PBS组明显升高,而IL-10水平较PBS组明显下降,差异均有统计学意义(P<0.05).大鼠骨髓间充质干细胞移植组在移植后1周、1个月、2个月及3个月时大鼠静脉血中IL-2水平较脐带间充质干细胞移植组明显升高,IL-10水平较对照组明显下降,差异均有统计学意义(P<0.05).三组在急性期(细胞移植后l周)移植局部均有大量CD3+细胞浸润.而移植的慢性期(细胞移植后3个月)大鼠骨髓间充质干细胞移植组仍存在大量CD3+细胞浸润,与其他两组相比差异均有统计学意义(P<0.05).结论 异体干细胞细胞移植后可发生急、慢性免疫排斥反应.HUCMSCs较大鼠BMSCs有更低的免疫源性,更利于脊髓损伤的功能恢复.
目的 觀察人臍帶間充質榦細胞(HUCMSCs)與大鼠骨髓間充質榦細胞(BMSCs)移植治療大鼠脊髓損傷後的功能恢複和急慢性排斥反應.方法 選取SD大鼠40隻按隨機數字錶隨機分成3組:臍帶間充質榦細胞移植組16隻、骨髓間充質榦細胞移植組16隻和PBS組8隻.採用脊髓半切手術製作大鼠脊髓損傷模型,損傷後對臍帶間充質榦細胞移植組進行HUCMSCs移植,對骨髓間充質榦細胞移植組行大鼠BMSCs移植,對PBS組移植同等劑量的PBS.通過BBB評分對各組動物的術後恢複進行評估比較.用ELISA法測定移植後3組動物不同時間點(移植後1週、1箇月、2箇月及3箇月)血中IL-2及IL-10的水平,併通過免疫組化的方法檢測移植跼部病理切片CD3+細胞浸潤的情況.結果 在移植後1週、1箇月、2箇月及3箇月時,檢測到榦細胞移植組各箇時間點大鼠靜脈血中IL-2水平較PBS組明顯升高,而IL-10水平較PBS組明顯下降,差異均有統計學意義(P<0.05).大鼠骨髓間充質榦細胞移植組在移植後1週、1箇月、2箇月及3箇月時大鼠靜脈血中IL-2水平較臍帶間充質榦細胞移植組明顯升高,IL-10水平較對照組明顯下降,差異均有統計學意義(P<0.05).三組在急性期(細胞移植後l週)移植跼部均有大量CD3+細胞浸潤.而移植的慢性期(細胞移植後3箇月)大鼠骨髓間充質榦細胞移植組仍存在大量CD3+細胞浸潤,與其他兩組相比差異均有統計學意義(P<0.05).結論 異體榦細胞細胞移植後可髮生急、慢性免疫排斥反應.HUCMSCs較大鼠BMSCs有更低的免疫源性,更利于脊髓損傷的功能恢複.
목적 관찰인제대간충질간세포(HUCMSCs)여대서골수간충질간세포(BMSCs)이식치료대서척수손상후적공능회복화급만성배척반응.방법 선취SD대서40지안수궤수자표수궤분성3조:제대간충질간세포이식조16지、골수간충질간세포이식조16지화PBS조8지.채용척수반절수술제작대서척수손상모형,손상후대제대간충질간세포이식조진행HUCMSCs이식,대골수간충질간세포이식조행대서BMSCs이식,대PBS조이식동등제량적PBS.통과BBB평분대각조동물적술후회복진행평고비교.용ELISA법측정이식후3조동물불동시간점(이식후1주、1개월、2개월급3개월)혈중IL-2급IL-10적수평,병통과면역조화적방법검측이식국부병리절편CD3+세포침윤적정황.결과 재이식후1주、1개월、2개월급3개월시,검측도간세포이식조각개시간점대서정맥혈중IL-2수평교PBS조명현승고,이IL-10수평교PBS조명현하강,차이균유통계학의의(P<0.05).대서골수간충질간세포이식조재이식후1주、1개월、2개월급3개월시대서정맥혈중IL-2수평교제대간충질간세포이식조명현승고,IL-10수평교대조조명현하강,차이균유통계학의의(P<0.05).삼조재급성기(세포이식후l주)이식국부균유대량CD3+세포침윤.이이식적만성기(세포이식후3개월)대서골수간충질간세포이식조잉존재대량CD3+세포침윤,여기타량조상비차이균유통계학의의(P<0.05).결론 이체간세포세포이식후가발생급、만성면역배척반응.HUCMSCs교대서BMSCs유경저적면역원성,경리우척수손상적공능회복.
Objective To observe changes in the functional recovery and immunological rejection of rats after spinal cord injuries with the treatment of transplanting human umbilical cord mesenchymal stem cells and rat bone marrow mesenchymal stem cells,to find the right stem cells for clinical procedures through this experiment,and to investigate the mechanism of immunological rejection after transplanting the cells.Methods Forty SD rats were selected randomly and divided into 3 groups.16 rats were placed in the umbilical cord mesenchymal stem cell transplantation group,16 rats in the bone marrow mesenchymal stem cell transplantation group,and 8 rats in the PBS group.The levels of 1I-2 and IL-10 in the blood of each group were detected at different times (1W and 2M,and 3 months after the cell transplantation) to reflect the systemic immunological rejection of the goat after transplantation.The CD3 + cells in the cell transplantation areas of each group were also detected with immunohistochemistry to reflect local immune rejection after transplantations.Results 1.The level of IL-2 was obviously higher than the PBS group lW and 2M and 3 months after the cell transplantation (P <0.05).Meanwhile,IL-10 was Contrary to the IL -2.2.The level of IL-2 was obviously higher than the PBS group 1W and 2M and 3 months after the cell transplantation (P < 0.05).Meanwhile,IL-10 was Contrary to the IL-2.3.The quantity of CD3+cells in all groups were much at the acute period (1 W after cell transplantation),but only the quantity of CD3+cells in the bone marrow mesenchymal stem cell transplantation group was much larger compared to the other groups at the chronic period 3 months after cell transplantation (P < 0.05).Conclusion Systemic and local immunological rejections at acute or chronic periods will appear in rats with spinal cord injuries that underwent stem cell transplantations.Compared with bone marrow mesenchymal stem cells,the umbilical cord mesenchymal stem cells significantly have lower immunogenicity and more suited for functional recoverv.