中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2013年
3期
255-258
,共4页
施金龙%陈建%倪兰春%施炜%徐锋%朱剑虹
施金龍%陳建%倪蘭春%施煒%徐鋒%硃劍虹
시금룡%진건%예란춘%시위%서봉%주검홍
神经胶质瘤%异柠檬酸脱氢酶1%突变%DNA甲基化
神經膠質瘤%異檸檬痠脫氫酶1%突變%DNA甲基化
신경효질류%이저몽산탈경매1%돌변%DNA갑기화
Glioma%IDH1%Mutation%DNA methylation
目的 分析星形细胞胶质瘤患者IDH1基因突变分布与病理级别、预后的关系;探讨DNA甲基化是否为IDH1基因调控的模式之一.方法 直接测序法检测91例星形细胞胶质瘤组织样本中IDH1的突变分布;重亚硫酸盐测序法检测9例星形细胞胶质瘤中IDH1启动子DNA甲基化水平.结果 91例星形细胞胶质瘤患者中35例存在IDH1突变(38.5%),均为R132H突变.WHO Ⅰ级肿瘤中未检测到IDH1突变;WHOⅡ~Ⅳ级原发性星形细胞胶质瘤中IDH1突变率分别为70.6%、62.1%、12.5%,突变率与病理级别呈负相关(r=0.522,P<0.001);7例继发性多形性胶质母细胞瘤中有5例(71.4%)发生突变,突变类型与原发肿瘤一致.IDH1突变型胶质瘤患者中位生存期较IDH1野生型患者明显延长(P<0.05).9例胶质瘤患者IDH1启动子均未检测到DNA甲基化改变.结论 IDH1突变在星形细胞胶质瘤中发生频繁,是预后改善的指标;DNA甲基化可能未参与IDH1基因表达的调控.
目的 分析星形細胞膠質瘤患者IDH1基因突變分佈與病理級彆、預後的關繫;探討DNA甲基化是否為IDH1基因調控的模式之一.方法 直接測序法檢測91例星形細胞膠質瘤組織樣本中IDH1的突變分佈;重亞硫痠鹽測序法檢測9例星形細胞膠質瘤中IDH1啟動子DNA甲基化水平.結果 91例星形細胞膠質瘤患者中35例存在IDH1突變(38.5%),均為R132H突變.WHO Ⅰ級腫瘤中未檢測到IDH1突變;WHOⅡ~Ⅳ級原髮性星形細胞膠質瘤中IDH1突變率分彆為70.6%、62.1%、12.5%,突變率與病理級彆呈負相關(r=0.522,P<0.001);7例繼髮性多形性膠質母細胞瘤中有5例(71.4%)髮生突變,突變類型與原髮腫瘤一緻.IDH1突變型膠質瘤患者中位生存期較IDH1野生型患者明顯延長(P<0.05).9例膠質瘤患者IDH1啟動子均未檢測到DNA甲基化改變.結論 IDH1突變在星形細胞膠質瘤中髮生頻繁,是預後改善的指標;DNA甲基化可能未參與IDH1基因錶達的調控.
목적 분석성형세포효질류환자IDH1기인돌변분포여병리급별、예후적관계;탐토DNA갑기화시부위IDH1기인조공적모식지일.방법 직접측서법검측91례성형세포효질류조직양본중IDH1적돌변분포;중아류산염측서법검측9례성형세포효질류중IDH1계동자DNA갑기화수평.결과 91례성형세포효질류환자중35례존재IDH1돌변(38.5%),균위R132H돌변.WHO Ⅰ급종류중미검측도IDH1돌변;WHOⅡ~Ⅳ급원발성성형세포효질류중IDH1돌변솔분별위70.6%、62.1%、12.5%,돌변솔여병리급별정부상관(r=0.522,P<0.001);7례계발성다형성효질모세포류중유5례(71.4%)발생돌변,돌변류형여원발종류일치.IDH1돌변형효질류환자중위생존기교IDH1야생형환자명현연장(P<0.05).9례효질류환자IDH1계동자균미검측도DNA갑기화개변.결론 IDH1돌변재성형세포효질류중발생빈번,시예후개선적지표;DNA갑기화가능미삼여IDH1기인표체적조공.
Objective To investigate the relationship between isocitrate dehydrogenase 1 (IDH1)gene mutation and the pathological grade and prognosis in astrocytic glioma patients.And to identify whether DNA methylation is one of the IDH1 gene regulatory patterns.Methods IDH1 mutation was detected by direct sequencing in 91 astrocytic glioma tissue samples; DNA methylation of ID-H1 promoter was detected by bisulfite sequencing in 9 astrocytic glioma cases.Results IDH1 mutations were demonstrated in 35 of 91 cases (38.5%) and all mutations were the type of R132H.No mutation of IDH1 was detected in WHO grade Ⅰ astrocytoma.Frequent mutations of IDH1 were observed as 70.6% in primary WHO grade Ⅱ astrocytoma,62.1% in primary WHO grade Ⅲ astrocytoma,12.5% in primary glioblastoma multiforme.Frequencies of IDH1 mutations were negatively correlated with WHO pathological grade Ⅱ ~ Ⅳ of primary astrocytic glioma (r =0.522,P <0.001).IDH1 mutations were found in 5 of 7 secondary glioblastomas (71.4%),and the type of mutation was consistent with that in primary tumors.The mutation of the IDH1 was significantly associated with better outcome in the patients of astrocytic gliomas (P < 0.05).DNA methylation of IDH1 promoter was not found in 9 glioma patients.Conclusions IDH1 mutation occured frequently in astrocytic gliomas and is a favorable prognostic indicator.DNA methylation might not be involved in the regulation of IDH1 gene expression.