CAJ | 학술논문

目的研究他达拉非增加脑淋巴瘤内微血管通透性.方法采用依文思蓝检测通透性、高效液相法检测氨甲喋呤浓度、以及电镜观察超微结构对比分析他达拉非对小鼠脑实质淋巴瘤模型脑微血管通透性的影响.结果他达拉非组脑淋巴瘤血管通透性相较其他各组有显著增加(P＜0.05).氨甲喋呤在他达拉非组脑淋巴瘤组织中浓度增加近1.8倍,显著高于其他各组(P＜0.05).他达拉非组小鼠脑淋巴瘤脑微血管内皮细胞内电镜下见大量微囊液泡.结论磷酸二酯酶抑制剂他达拉非可以显著增加小鼠脑淋巴瘤微血管的通透性.
목적 연구타체랍비증가뇌림파류내미혈관통투성.방법 채용의문사람검측통투성、고효액상법검측안갑첩령농도、이급전경관찰초미결구대비분석타체랍비대소서뇌실질림파류모형뇌미혈관통투성적영향.결과 타체랍비조뇌림파류혈관통투성상교기타각조유현저증가(P＜0.05).안갑첩령재타체랍비조뇌림파류조직중농도증가근1.8배,현저고우기타각조(P＜0.05).타체랍비조소서뇌림파류뇌미혈관내피세포내전경하견대량미낭액포.결론 린산이지매억제제타체랍비가이현저증가소서뇌림파류미혈관적통투성.
Objective To study about Tadalafil increasing microvascular permeability in mouse brain lymphoma models.Methods Comparatively analyze the influence of tadalafil on cerebral microvascular permeability in the mouse brain lymphoma models,by the approaches of using Evan's Blue to test the microvascular permeability,testing concentrations of methotrexate (MTX) by HPLC,and studying ultrastructures by transmission electron microscope.Results The microvascular permeability in tumor-bearing mice treated with Tadalafil were significantly higher than that in the other groups (P ＜ 0.05).The concentration of MTX increased nearly 1.8 times in tumor tissue with Tadalafil pre-treatment.The increment were significantly higher than that in the other groups (P ＜ 0.05).Numerous pinocytotic vesicles in the cytoplasm of the endothelial cells were observed obviously in Tadalafil pre-treated mouse tumor.Conclusion Phosphodiesterase type 5 inhibitor,Tadalafil,could increased the capillary permeability of the tumor tissue in mouse brain lymphoma models.