CAJ | 학술논문

目的探讨ADAM10基因mRNA及蛋白在胶质瘤细胞中的表达及生物学意义.方法我院2007年6月至2010年7月共计43例脑胶质瘤患者标本,低级别胶质瘤22例,高级别胶质瘤21例,取脑膜瘤5例作为阴性对照组,采用RT-PCR、免疫组织化学实验方法检测ADAM10基因及蛋白的表达,进行半定量分析和细胞形态学观察.结果单因素方差分析三组平均灰度值结果如下:阴性对照组为4.8,低级别组为22.1,高级别组为32.3,高级别组和低级别组比较差异有统计学意义(P＜0.05),低级别组和阴性对照组比较差异有统计学意义(P＜0.01)；免疫组化染色提示蛋白主要定位于肿瘤细胞膜、瘤组织内血管壁上.结论胶质瘤恶性度越高,肿瘤侵袭性越强,ADAM10表达也越多,推测其可能参与胶质瘤侵袭生长；ADAM10同时也在血管壁上有较多表达,推测其可能与瘤周水肿形成有关.
목적 탐토ADAM10기인mRNA급단백재효질류세포중적표체급생물학의의.방법 아원2007년6월지2010년7월공계43례뇌효질류환자표본,저급별효질류22례,고급별효질류21례,취뇌막류5례작위음성대조조,채용RT-PCR、면역조직화학실험방법검측ADAM10기인급단백적표체,진행반정량분석화세포형태학관찰.결과 단인소방차분석삼조평균회도치결과여하:음성대조조위4.8,저급별조위22.1,고급별조위32.3,고급별조화저급별조비교차이유통계학의의(P＜0.05),저급별조화음성대조조비교차이유통계학의의(P＜0.01)；면역조화염색제시단백주요정위우종류세포막、류조직내혈관벽상.결론 효질류악성도월고,종류침습성월강,ADAM10표체야월다,추측기가능삼여효질류침습생장；ADAM10동시야재혈관벽상유교다표체,추측기가능여류주수종형성유관.
Objective To investigate the expression and significance of ADAM10 mRNA and protein in human glioma.Methods Forty-three cases with glioma undergoing surgical treatment in our hospital from 2007 to 2010 were choosed as trial sample and fivety cases with meningioma were treated as control.Reverse transcription polymerase chain reaction (RT-PCR),and immunohistochemistry were performed to detect the expressions of ADAM10 mRNA and protein in all the samples.Results the expression of ADAM10 mRNA in meningioma group and in low grade are significantly lower than those in high grade group(P ＜0.01;P ＜0.05).The ADAM10 are located in the surface of glioma cell and blood vessel in the tumor tissue.Conclusions ADAM10 are associated with partial biological behaviors,which may be involved in the development of glioma and edema.