CAJ | 학술논문

目的探讨大鼠C6脑胶质瘤模型在高场强动物MRI的影像学特征.方法建立Wistar大鼠C6脑胶质瘤模型,2周后行高场强MRI及功能成像序列扫描,同时获取脑胶质瘤标本做病理学检查.结果成功建立大鼠C6脑胶质瘤模型,病理学类似人脑胶质瘤.常规序列成像呈长T1,长T2信号,强化明显；MRI弥散成像呈稍高信号,肿瘤区域表观弥散信号较止常组织偏高；氢质子波谱成像上胶质瘤组织与正常组织相比N-乙酰天门冬氨酸峰值明显减低,胆碱峰值升高,肌酸峰值轻度降低；灌注图像脑肿瘤实质区域趋于低灌注,肿瘤周边区域见异常稍高灌注区.结论成功获得大鼠C6脑胶质瘤在高场强动物MRI的影像形态学及功能成像信息,为动物胶质瘤实验研究提供影像学依据.
목적 탐토대서C6뇌효질류모형재고장강동물MRI적영상학특정.방법 건립Wistar대서C6뇌효질류모형,2주후행고장강MRI급공능성상서렬소묘,동시획취뇌효질류표본주병이학검사.결과 성공건립대서C6뇌효질류모형,병이학유사인뇌효질류.상규서렬성상정장T1,장T2신호,강화명현；MRI미산성상정초고신호,종류구역표관미산신호교지상조직편고；경질자파보성상상효질류조직여정상조직상비N-을선천문동안산봉치명현감저,담감봉치승고,기산봉치경도강저；관주도상뇌종류실질구역추우저관주,종류주변구역견이상초고관주구.결론 성공획득대서C6뇌효질류재고장강동물MRI적영상형태학급공능성상신식,위동물효질류실험연구제공영상학의거.
Objective To establish C6 rat brain glioma model and study its features on high field animal magnetic resonance imaging (MRI).Methods Twenty wistar rats with body weight range from 250 g to 270 g were chosen and 1 × 106glioma cells were injected stereotactically into the right caudate nucleus of the brain to establish rat brain glioma models.2 weeks after the implantation,all rats were examined with high field animal MRI,including routine MR imaging,Diffusion weighted imaging (DWI),proton magnetic resonance spectroscopy (1 H-MRS) and Pcrfusion weighted imaging (PWI).After MRI examination,the rats were sampled for H&E staining and immunohistochemical staining.Results After 2 weeks growth of the tumor,the rate of successfully developing rat brain glioma was 100％.C6 rat brain gliomas were proved similarly to human glioma by pathology.The golimas were long T1 and long T2 signal on routine MRI sequence,high signal on DWI sequence and the apparent diffusion coefficient (ADC) signal of the tumor tissues was higher than normal tissues.In the 1H-MRS imaging,compared with the normal tissue,the value of N-acetyl aspartic acid (NAA) was apparently decreased in the tumor area,with Choline increased,and creatine slightly decrcased.The original PWI images were adjusted and got rCBV imaging、rCBF imaging and MTT imaging.The tumor area was low perfusion with high perfusion in some surrounding areas.Conclusion The model of C6 rat brain glioma can be established stereotactically and obtain routine and functional image in high field animal MRI successfully,which will provide more MR imaging message for animal experiment of glioma in the future.