CAJ | 학술논문

目的探讨肿瘤坏死因子凋亡相关诱导配体(TRAIL)与硝普钠(SNP)联合诱导U251细胞凋亡的协同作用.方法将TRAIL和SNP单药或联合作用于U251胶质瘤细胞系.MTT比色法、流式细胞术检测细胞生长抑制率、凋亡率；Western blot法检测DR5、Caspase-3、Bcl-2、Survivin 蛋白表达.结果 TRAIL和SNP单药组对U251细胞活性均有抑制作用；联合作用组细胞凋亡率明显高于单药组和对照组(P＜0.01),并存在协同作用；联合作用组较对照组DR5、Caspase-3蛋白表达明显上调,Survivin、Bcl-2蛋白表达明显下调(P＜0.01).结论 TRAIL和SNP可显著抑制U251细胞增殖,具有协同细胞凋亡诱导作用.其机制与上调DR5、Caspase-3,下调Bcl-2、Survivin 蛋白表达有关.
목적 탐토종류배사인자조망상관유도배체(TRAIL)여초보납(SNP)연합유도U251세포조망적협동작용.방법 장TRAIL화SNP단약혹연합작용우U251효질류세포계.MTT비색법、류식세포술검측세포생장억제솔、조망솔；Western blot법검측DR5、Caspase-3、Bcl-2、Survivin 단백표체.결과 TRAIL화SNP단약조대U251세포활성균유억제작용；연합작용조세포조망솔명현고우단약조화대조조(P＜0.01),병존재협동작용；연합작용조교대조조DR5、Caspase-3단백표체명현상조,Survivin、Bcl-2단백표체명현하조(P＜0.01).결론 TRAIL화SNP가현저억제U251세포증식,구유협동세포조망유도작용.기궤제여상조DR5、Caspase-3,하조Bcl-2、Survivin 단백표체유관.
Objective To investigate the synergistic apoptotic effect of TNF-Related Apoptosis,inducing Ligand (TRAIL) combined with sodium nitroprusside (SNP).Methods U251 glioma cell line was treated with TRAIL,SNP respectively and their combination.The inhibition rate of U251 cell line was detected with MTT assay,and the apoptotic rate was analyzed with flow cytometry.The protein expressions of DR5、Caspase-3、Bcl-2、survivin were detected with western blot.Results Inhibitory effect of U251 cell line was observed in both TRAIL and SNP groups,and the combination group represented significantly higher inhibition rate and apoptotic rate.Synergistic apoptosis inducing effect was observed in the combination group (P ＜0.01).Compared with control,the expression of Caspase-3 was higher,while that of Bcl-2 was lower in both groups,and the expression of DR5 was higher while that of survivin was lower in the SNP group (P ＜0.01).Conclusions TRAIL and SNP can inhibit the proliferation and induce apoptosis of U251 cell line.Their combination has a synergistic effect which may due to the up-regulation of DR5 and Caspase -3 and down-regulation of Bcl-2 and survivin.