CAJ | 학술논문

目的探索组蛋白去乙酰化酶3(HDAC3)基因对胶质瘤细胞U87-MG增殖、凋亡、细胞周期以及迁移的作用.方法人胶质瘤细胞株U87-MG,应用siRNA干扰技术分别导入HDAC3干扰质粒和空载对照质粒,蛋白印迹实验(Western blot)检测干扰效果,应用CCK-8增殖实验、成球实验、Annexin V-FITC细胞凋亡试剂盒、碘化丙啶染色方法、Transwell小室实验和划痕实验,分别检测HDAC3基因干扰后对胶质瘤细胞株增殖、凋亡、细胞周期和迁移等功能的影响.结果 Western blot 结果显示,干扰后细胞株U87-MG中的HDAC3表达明显减弱；CCK-8增殖实验和成球实验,与对照组相比较,胶质瘤细胞株中干扰HDAC3后细胞增殖曲线明显下降,U87-MG对照组和U87-MG Si的14 d成球总数目分别为23和7(P＜0.05)；细胞凋亡实验,U87-MG对照组和U87-MG Si的早期凋亡率分别为(1.40±0.29)％和(9.26±2.32)％(P=0.004)；周期实验,U87-MG对照组和U87-MG Si的Go/G1期百分率分别为80.34％和88.37％,(t检验,P＜0.05)；Transwell小室实验和划痕实验,胶质瘤细胞株U87-MG中干扰HDAC3后细胞迁移能力明显下降.结论 HDAC3作为一种致癌基因,通过调控细胞Go/G1期参与胶质瘤细胞的增殖、凋亡和迁移.
목적 탐색조단백거을선화매3(HDAC3)기인대효질류세포U87-MG증식、조망、세포주기이급천이적작용.방법 인효질류세포주U87-MG,응용siRNA간우기술분별도입HDAC3간우질립화공재대조질립,단백인적실험(Western blot)검측간우효과,응용CCK-8증식실험、성구실험、Annexin V-FITC세포조망시제합、전화병정염색방법、Transwell소실실험화화흔실험,분별검측HDAC3기인간우후대효질류세포주증식、조망、세포주기화천이등공능적영향.결과 Western blot 결과현시,간우후세포주U87-MG중적HDAC3표체명현감약；CCK-8증식실험화성구실험,여대조조상비교,효질류세포주중간우HDAC3후세포증식곡선명현하강,U87-MG대조조화U87-MG Si적14 d성구총수목분별위23화7(P＜0.05)；세포조망실험,U87-MG대조조화U87-MG Si적조기조망솔분별위(1.40±0.29)％화(9.26±2.32)％(P=0.004)；주기실험,U87-MG대조조화U87-MG Si적Go/G1기백분솔분별위80.34％화88.37％,(t검험,P＜0.05)；Transwell소실실험화화흔실험,효질류세포주U87-MG중간우HDAC3후세포천이능력명현하강.결론 HDAC3작위일충치암기인,통과조공세포Go/G1기삼여효질류세포적증식、조망화천이.
Objective To explore the relationship between histone deacetylase 3 (HDAC3)and growth,apoptosis,cell cycle and migration of glioma cells U87-MG.Methods Western blot were used to detect the expression of HDAC3 in glioma cells U87-MG after transfected with siRNA and control RNA.CCK-8 cell proliferation assay,sphere formation,apoptosis assay,transwell migration assay and monolayer wound healing assay were used to explore the effect of the cell growth,apoptosis,cell cycle and migration.Results Western blot results showed that after transfected with siRNA,the expression of HDAC3 in glioma cells were significantly lower.The result of cell proliferation assay showed cell growth of siRNA groups declined compared with control groups.14 days sphere numbers of U87-MG control group and U87-MG Si group was 23 and 7(P ＜0.05).The early apoptosis rate of U87-MG control group and Si group were (1.40 ±0.29) ％ and (9.26 ±2.32) ％ (P =0.004).G0/Gt of U87-MG group and Si group were 80.34％ and 88.37％.Transwell migration assay and monolayer wound healing assay also declared that the migration became weaker after transfected.Conclusions HDAC3 participated in cell growth,apoptosis,migration of glioma cells through G0/G1 arrest as an oncogene.