CAJ | 학술논문

目的探讨高迁移率族蛋白A2 (HMGA2)和基质金属蛋白酶(MMP-2)在星形细胞瘤中的表达与侵袭性、病理学分型的关系及HMGA2和MMP-2之间的相互关系.方法应用免疫组化(S-P)方法对78例星形细胞瘤组织中的HMGA2和MMP-2进行检测.应用实时荧光定量聚合酶链反应(qPCR)检测HMGA2和MMP-2的mRNA表达.结果 78例星形细胞瘤中HMGA2和MMP-2的蛋白阳性率分别为92.30％和89.74％,HMGA2基因表达水平在胶质母细胞瘤(2.56倍,P＜ 0.01)和间变性星形细胞瘤(1.98倍,P＜0.01)中显著高于星形细胞瘤,而在胶质母细胞瘤与间变性星形细胞瘤之间无显著区别(1.29倍,P=0.118).HMGA2与MMP-2之间的蛋白及mRNA表达水平存在相关关系(P＜0.01).结论 HMGA2及MMP-2的增强表达与星形细胞瘤恶性程度增高及肿瘤侵袭性增强有密切关系,其表达可作为判断星形细胞瘤患者预后的指标.
목적 탐토고천이솔족단백A2 (HMGA2)화기질금속단백매(MMP-2)재성형세포류중적표체여침습성、병이학분형적관계급HMGA2화MMP-2지간적상호관계.방법 응용면역조화(S-P)방법대78례성형세포류조직중적HMGA2화MMP-2진행검측.응용실시형광정량취합매련반응(qPCR)검측HMGA2화MMP-2적mRNA표체.결과 78례성형세포류중HMGA2화MMP-2적단백양성솔분별위92.30％화89.74％,HMGA2기인표체수평재효질모세포류(2.56배,P＜ 0.01)화간변성성형세포류(1.98배,P＜0.01)중현저고우성형세포류,이재효질모세포류여간변성성형세포류지간무현저구별(1.29배,P=0.118).HMGA2여MMP-2지간적단백급mRNA표체수평존재상관관계(P＜0.01).결론 HMGA2급MMP-2적증강표체여성형세포류악성정도증고급종류침습성증강유밀절관계,기표체가작위판단성형세포류환자예후적지표.
Objective To probe into the expression of high mobility group A2 (HGMA2) and matrix metalloproteinase-2 (MMP-2) in low-grade and high-grade astrocyte tumors and their correlation with invasion,pathological classification and their reciprocal relationship.Methods The expression of HMGA2 and MMP-2 was examined by immunohistochemical staining method (S-P).The mRNA expression of HMGA2 and MMP-2 was examined with RT-PCR.Results The positive cell rates of HMGA2 and MMP-2 in 78 astrocyte tumors were 92.30％ and 89.74％,respectively,and the degree of positive cells was in accordance with the malignancy of tumors.The QPCR results showed that the expression of HMGA2 protein was significantly higher in glioblastoma multiforme (2.56 fold,P ＜ 0.01) and anaplastic astrocytoma(1.98 fold,P ＜ 0.01) tumors than that in diffuse astrocytoma.There was correlation between HMGA2 and MMP-2.Conclusions The up-regulated expression level of HMGA2 and MMP-2 was closely related to the increased malignancy and invasion of tumors.The expression of protein and mRNA might act as an indicator the prognosis of patients with astrocyte tumors.