CAJ | 학술논문

目的对比动物模型动静脉畸形(AVM)和人脑动静脉畸形(CAVM)的形态特征,证明大鼠模型AVM与人脑AVM血管形态学上的相似性.方法此次观察研究了17例人类动静脉畸形的标本和71只动静脉畸形的大鼠模型的标本,应用组织学方法观察了血管的大体结构,同时利用流量探针测量并比较两组血管的血流量.结果大鼠动静脉畸形模型和的人脑动静脉畸形标本中都能够观察到不连续的血脑屏障,内皮细胞中断,内皮细胞有大量丝状伪足、窗孔形成、溶酶体、胞质囊泡及空泡等相似的组织学结构.大鼠动静脉畸形的血流速度较正常明显加快,并于第42 d时达到峰值(P＜0.05).结论大鼠动静脉畸形模型与人类动静脉畸形具有相似的血流动力学、形态学及超微结构特征,因此为利用此模型研究AVM奠定了基础.
목적 대비동물모형동정맥기형(AVM)화인뇌동정맥기형(CAVM)적형태특정,증명대서모형AVM여인뇌AVM혈관형태학상적상사성.방법 차차관찰연구료17례인류동정맥기형적표본화71지동정맥기형적대서모형적표본,응용조직학방법관찰료혈관적대체결구,동시이용류량탐침측량병비교량조혈관적혈류량.결과 대서동정맥기형모형화적인뇌동정맥기형표본중도능구관찰도불련속적혈뇌병장,내피세포중단,내피세포유대량사상위족、창공형성、용매체、포질낭포급공포등상사적조직학결구.대서동정맥기형적혈류속도교정상명현가쾌,병우제42 d시체도봉치(P＜0.05).결론 대서동정맥기형모형여인류동정맥기형구유상사적혈류동역학、형태학급초미결구특정,인차위이용차모형연구AVM전정료기출.
Objective To compare the blood flow and histological changes between arteriovenous malformations in a rat model and human cerebral arteriovenous malformations.Methods Seventeen human specimens of arteriovenous malformations specimens and 71 samples of arteriovenous malformations were randomly selected.All specimens were examined histologically,immunohistologically,and electromicroscopically.Blood flow was measured using a flow probe connected to a transit perivascular flowmeter.Results Gap junctions were observed between endothelial cells in both human arteriovenous malformation specimens and rat model.There were numerous filopodia,fenestrated processes,lysosomes,cytoplasmic vesicles and vacuoles observed in human arteriovenous malformations and the nidus of model.The blood flow increased in the arterialized vein in rat model,and the flow rate peaked at 42 days post fistula formation(P ＜ 0.05).Conclusion The rat AVM model demonstrated similar hemodynamic,morphological,and ultrastructural features to human AVM,thus this model would be suitable for use in studying arteriovenous malformations.