CAJ | 학술논문

目的探讨多配体蛋白聚糖结合蛋白(Syntenin)促进脑胶质瘤迁移和侵袭过程的分子机制.方法应用RT-PCR技术和免疫印迹方法研究84例不同病理级别人脑胶质瘤组织中Syntenin与磷酸化FAK Tyr397基因和蛋白的表达情况,比较二者与胶质瘤分级的关系以及表达的相关性.结果 RT-PCR检测结果显示,Syntenin mRNA和FAK mRNA表达水平在各级胶质瘤组织之间均存在明显差异(P＜0.05),二者表达水平的上调均与肿瘤级别的增加呈正相关(r Syntenin=0.91,P＜ 0.05; rFAK =0.97,P＜0.05);免疫印迹结果发现,Syntenin和磷酸化FAK Tyr397的表达在各级胶质瘤组织中存在差异(P＜0.05);其表达水平均随胶质瘤病理级别的增加而上调(r Syntenin=0.91,P＜ 0.05;rFAK=0.90,P＜0.05);Syntenin表达水平的提高与FAK Tyr397的磷酸化水平呈正相关(r =0.90,P＜0.05).结论脑胶质瘤细胞中Syntenin可能通过与FAK相互作用经相应的信号转导途径增加脑胶质瘤细胞迁移和侵袭的能力.
목적 탐토다배체단백취당결합단백(Syntenin)촉진뇌효질류천이화침습과정적분자궤제.방법 응용RT-PCR기술화면역인적방법연구84례불동병리급별인뇌효질류조직중Syntenin여린산화FAK Tyr397기인화단백적표체정황,비교이자여효질류분급적관계이급표체적상관성.결과 RT-PCR검측결과현시,Syntenin mRNA화FAK mRNA표체수평재각급효질류조직지간균존재명현차이(P＜0.05),이자표체수평적상조균여종류급별적증가정정상관(r Syntenin=0.91,P＜ 0.05; rFAK =0.97,P＜0.05);면역인적결과발현,Syntenin화린산화FAK Tyr397적표체재각급효질류조직중존재차이(P＜0.05);기표체수평균수효질류병리급별적증가이상조(r Syntenin=0.91,P＜ 0.05;rFAK=0.90,P＜0.05);Syntenin표체수평적제고여FAK Tyr397적린산화수평정정상관(r =0.90,P＜0.05).결론 뇌효질류세포중Syntenin가능통과여FAK상호작용경상응적신호전도도경증가뇌효질류세포천이화침습적능력.
Objective To investigate the molecular mechanism of Syntenin in the enhancement of migration and invasion of brain gliomas.Methods We investigated the expression levels of Syntenin mRNA and FAK mRNA with reverse transcription-polymerase chain reaction (RT-PCR),and verifed the expression levels of Syntenin and phosphorylated FAK Tyr397 with immunoblot in 84 cases of human brain gliomas.Its correlation to the pathologic grade,and the relationship between the expression of Syntenin and phosphorylated FAK Tyr397 in human brain gliomas were studied.Results There were significant differences (P ＜ 0.05) in the expression of Syntenin mRNA and FAK mRNA in glioma tissues by RT-PCR.The increasing expression levels of Syntenin mRNA and FAK mRNA had positive correlation with the ascending of pathologic grade of tumor specimen (r Syntenin =0.91,P ＜ 0.05,r FAK =0.97,P ＜ 0.05).The results of immunoblot showed that there were significant differences (P ＜ 0.05) in the expression of Syntenin and phosphorylated FAK Tyr397 in glioma tissues.With the ascending of pathologic grade of tumor specimens,the expression levels of Syntenin and phosphorylated FAK Tyr397 protein increased markedly(r Syntenin =0.91,P ＜ 0.05; r FAK =0.90,P ＜ 0.05).There was positive correlation betwenn the expression level of phosphorylated FAK Tyr397 and Syntenin in gliomas (r =0.90,P ＜ 0.05).Conclusion The invasion and migration ability of glioma might be enhanced by Syntenin though its interaction with FAK in a corresponding signal transduction pathway.