CAJ | 학술논문

目的探讨基因工程化神经移植物结合神经营养因子-3(NT-3)对C7脊神经背根损伤的治疗效果.方法 24只大鼠分为三组:A组:健康对照组,不做任何处理;B组:修复术组;C组:修复术结合基因治疗组.修复术4个月后采用热板实验检测热痛觉功能情况;神经电生理检测体感诱发电位(SSEP);逆行示踪检测神经纤维再生情况;电镜观察髓鞘再生情况.结果修复术4个月后,B组和C组大鼠损伤侧前肢热痛觉部分恢复,B组和C组患侧前肢回缩时间比对照组显著缩短(P＜0.01);神经电生理检测C组SSEP波幅和波面积明显高于B组,差异具有统计学意义(P＜0.01);逆向示踪显示C组中FG阳性或FG和CTB-Alexa 555双阳性神经元个数明显多于D组,差异有统计学意义(P＜0.01);电镜结果显示C组神经移植物中髓鞘更为致密,板层更厚,而B组神经纤维变性改变较C组明显.结论基因工程化的神经移植物,可促进受损C7背根神经节神经元的中枢突再生进入脊髓后角,加快损伤肢体感觉功能恢复.
목적 탐토기인공정화신경이식물결합신경영양인자-3(NT-3)대C7척신경배근손상적치료효과.방법 24지대서분위삼조:A조:건강대조조,불주임하처리;B조:수복술조;C조:수복술결합기인치료조.수복술4개월후채용열판실험검측열통각공능정황;신경전생리검측체감유발전위(SSEP);역행시종검측신경섬유재생정황;전경관찰수초재생정황.결과 수복술4개월후,B조화C조대서손상측전지열통각부분회복,B조화C조환측전지회축시간비대조조현저축단(P＜0.01);신경전생리검측C조SSEP파폭화파면적명현고우B조,차이구유통계학의의(P＜0.01);역향시종현시C조중FG양성혹FG화CTB-Alexa 555쌍양성신경원개수명현다우D조,차이유통계학의의(P＜0.01);전경결과현시C조신경이식물중수초경위치밀,판층경후,이B조신경섬유변성개변교C조명현.결론 기인공정화적신경이식물,가촉진수손C7배근신경절신경원적중추돌재생진입척수후각,가쾌손상지체감각공능회복.
Objective To explore the therapeutic effect of the transduction of Neurotrophin 3 gene into peripheral nerve graft for treatment of cervical dorsal root injury in adult rats.Methods 24 adult male Fisher 344 rats were grouped into A (intact),B (reconstruction with PNG-GFP),and C (reconstruction with PNG-NT-3).The treatment was assessed by hot plate test,electrophysiological examination,light and electron microscopy and neurotrace labeling examinations 4 months after the nerve reconstruction.Results Four months after reconstruction,partial pain perception defects were restored.Recording of somatosensory evoked potentials,axonal regeneration and fluorescent tracer transport showed electrical and physical reconnection of the C7 dorsal root ganglion neurons to the spinal cord through the reconstructed pathway in NT-3 and GFP groups.The results of electrophysiological examination and neurotracing in the NT-3 group were superior to the GFP group and the control group (P ＜ 0.01).Conclusions Local released NT-3 by genetic engineering peripheral nerve graft could promote the regeneration of injuried central axons of C7 dorsal root ganglion neurons into the posterior horn in adult rats.Partial sensory function defects were restored.