CAJ | 학술논문

目的探讨膜联蛋白A1(AnnexinA1)、脂蛋白相关磷脂酶A2(Lp-PLA2)和Bax与人颈动脉粥样硬化斑块形成及稳定性的关系. 方法选择郑州大学第五附属医院血管外科自2010年5月至2011年6月行颈动脉内膜剥脱术(CEA)的颈动脉粥样硬化狭窄(＞70％)患者45例作为颈动脉粥样硬化组,根据术前颈动脉超声检查结果再分为软斑组、混合斑组、硬斑组,每组15例；选取郑州大学第一附属医院同期外科手术切除的正常肠系膜动脉标本20例作为标本对照组；选取社区健康志愿者20例作为健康对照组.酶联免疫法(ELISA)测定颈动脉粥样硬化组和健康对照组血清中Lp-PLA2的含量；反转录-聚合酶链反应(RT-PCR)和Western blotting分别检测颈动脉粥样硬化组和标本对照组中AnnexinA1、Bax mRNA和蛋白的表达. 结果与健康对照组相比,软斑组、混合斑组、硬斑组患者血清中Lp-PLA2含量均增高,而且软斑组Lp-PLA2的含量高于硬斑组,差异有统计学意义(P＜0.05)；与标本对照组相比,软斑组、混合斑组、硬斑组Annexin A1、Bax mRNA及蛋白表达水平均上调,而且软斑组Annexin A1 mRNA及蛋白表达水平低于硬斑组,Bax mRNA及蛋白表达水平高于硬斑组,差异均有统计学意义(P＜0.05). 结论 Annexin A1、Lp-PLA2和Bax参与了人颈动脉粥样硬化斑块的形成及稳定.
목적 탐토막련단백A1(AnnexinA1)、지단백상관린지매A2(Lp-PLA2)화Bax여인경동맥죽양경화반괴형성급은정성적관계. 방법 선택정주대학제오부속의원혈관외과자2010년5월지2011년6월행경동맥내막박탈술(CEA)적경동맥죽양경화협착(＞70％)환자45례작위경동맥죽양경화조,근거술전경동맥초성검사결과재분위연반조、혼합반조、경반조,매조15례；선취정주대학제일부속의원동기외과수술절제적정상장계막동맥표본20례작위표본대조조；선취사구건강지원자20례작위건강대조조.매련면역법(ELISA)측정경동맥죽양경화조화건강대조조혈청중Lp-PLA2적함량；반전록-취합매련반응(RT-PCR)화Western blotting분별검측경동맥죽양경화조화표본대조조중AnnexinA1、Bax mRNA화단백적표체. 결과 여건강대조조상비,연반조、혼합반조、경반조환자혈청중Lp-PLA2함량균증고,이차연반조Lp-PLA2적함량고우경반조,차이유통계학의의(P＜0.05)；여표본대조조상비,연반조、혼합반조、경반조Annexin A1、Bax mRNA급단백표체수평균상조,이차연반조Annexin A1 mRNA급단백표체수평저우경반조,Bax mRNA급단백표체수평고우경반조,차이균유통계학의의(P＜0.05). 결론 Annexin A1、Lp-PLA2화Bax삼여료인경동맥죽양경화반괴적형성급은정.
Objective To investigate the correlation between levels of Annexin A1,Bax and lipoprotein-associated phospholipase A2 (Lp-PLA2) and both formation and stability of human carotid atherosclerotic plaques.Methods Forty-five specimens from patients with carotid atherosclerotic plaques (test group,group A),admitted to our hospital from May 2010 to June 2011 and performed carotid endarterectomy (CEA),and 20 specimens from patients with mesenteric artery and 20 healthy subjects (control groups,group B and group C,respectively) were collected in our study.The carotid atherosclerotic plaque specimens were divided into soft plaque group A1 (n=15),mixed plaque group A2 (n=15) and hard plaque group A3 (n=15) according to the results of carotid artery ultrasound.The Lp-PLA2 level in group A and group C was measured by enzyme linked immunosorbent assay (ELISA),and the mRNA and protein expressions of Annexin A1 and Bax in group A and group B were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.Results The level of Lp-PLA2 in all patients with carotid atherosclerotic plaques was significantly increased as compared with that in group C (P＜0.05); that in group A1 was significantly higher than that in group A3 (P＜0.05).The mRNA and protein expressions of Annexin A1 and Bax in all patients with carotid atherosclerotic plaques were significantly increased as compared with those in group B (P＜0.05); group A1 exhibited lower mRNA and protein expressions of Annexin A1 than group A3 (P＜0.05),while group A1 exhibited higher mRNA and protein expressions of Bax than group A3 (P＜0.05).Conclusion Annexin A1,Lp-PLA2 and Bax participate in the formation and stability of human carotid atherosclerotic plaques.