CAJ | 학술논문

目的探讨26S蛋白酶体的PSMB4亚基(PSMB4)在正常脑组织与胶质瘤组织中表达的差异性及其与脑胶质瘤恶性程度的相关性. 方法选取厦门大学附属第一医院神经外科自2008年1月至2012年2月间手术切除并经病理证实的人脑胶质瘤液氮冻存标本75例,其中星形细胞瘤Ⅰ级21例,星形细胞瘤Ⅱ级21例,星形细胞瘤Ⅲ级27例,胶质母细胞瘤Ⅳ级6例.另取5例因脑创伤行内减压术患者的正常脑组织液氮冻存标本,免疫组织化学染色检测标本中PSMB4的表达. 结果 PSMB4在正常脑组织中呈阴性表达,而在胶质瘤组织中呈阳性或强阳性表达.统计分析显示胶质瘤组PSMB4蛋白的表达明显高于正常脑组织组,差异有统计学意义(P＜0.05),不同级别胶质瘤PSMB4表达的分布差异无统计学意义(P＞0.05). 结论脑胶质瘤组织PSMB4蛋白的表达高于正常脑组织,但与脑胶质瘤的恶性程度无明显相关性.
목적 탐토26S단백매체적PSMB4아기(PSMB4)재정상뇌조직여효질류조직중표체적차이성급기여뇌효질류악성정도적상관성. 방법 선취하문대학부속제일의원신경외과자2008년1월지2012년2월간수술절제병경병리증실적인뇌효질류액담동존표본75례,기중성형세포류Ⅰ급21례,성형세포류Ⅱ급21례,성형세포류Ⅲ급27례,효질모세포류Ⅳ급6례.령취5례인뇌창상행내감압술환자적정상뇌조직액담동존표본,면역조직화학염색검측표본중PSMB4적표체. 결과 PSMB4재정상뇌조직중정음성표체,이재효질류조직중정양성혹강양성표체.통계분석현시효질류조PSMB4단백적표체명현고우정상뇌조직조,차이유통계학의의(P＜0.05),불동급별효질류PSMB4표체적분포차이무통계학의의(P＞0.05). 결론 뇌효질류조직PSMB4단백적표체고우정상뇌조직,단여뇌효질류적악성정도무명현상관성.
Objective To investigate the differences of proteasome subunit beta type-4 (PSMB4) expression between normal cerebral tissues and glioma tissues,and the relevance between its expression and glioma malignancy degree.Methods Seventy-five human glioma liquid nitrogen frozen specimens,collected and confirmed by pathology in our hospital from January 2008 to February 2012,including 21 with grade Ⅰ astrocytoma,21 with grade Ⅱ astrocytoma,27 with grade Ⅲ astrocytoma and 6 with grade Ⅳ glioblastoma,were chosen in our study; another 5 normal cerebral tissues from cerebral trauma patients performed decompression were chosen as controls.Immunohistochemistry wax employed to detect the PSMB4 expressions in both normal cerebral tissues and glioma tissues.Results PSMB4 protein expression was negative in the controls,while positive/strong positive in the gliomas.Statistical analysis showed that the PSMB4 protein expression level in the gliomas was significantly higher than that in the controls (P＜0.05).PSMB4 protein expression in gliomas of different degrees showed no statistically significant differences (P＞0.05).Conclusion PSMB4 protein expression in the glioma tissues is higher than that in the normal cerebral tissues,however,no relevance between PSMB4 expression and glioma malignancy grade is noted.