CAJ | 학술논문

目的评价神经节苷脂对糖尿病脑病大鼠模型认知功能的作用,并探讨其影响机制. 方法 60只大鼠按随机数字表法分为正常对照组、糖尿病脑病组和糖尿病脑病+神经节苷脂治疗组3组,每组再根据观察时间点不同分为1月、3月两亚组(A1、B1、C1、A3、B3、C3组),每亚组各10只.采用链脲佐菌素建立大鼠糖尿病脑病模型,糖尿病脑病+神经节苷脂治疗组每天腹腔注射神经节苷脂10 mg/kg.应用水迷宫行为学测试观察各组大鼠认知功能改变,免疫组化染色、RT-PCR检测海马区神经元特异性烯醇化酶(NSE)和S-100B蛋白及mRNA的表达. 结果 (1)A1、B1、C1组水迷宫行为学测试成绩分别为(6.7±0.5)s、(14.1±1.7)s、(6.9±0.6)s,A3、B3、C3组测试成绩分别为(7.0±0.5)s、(26.6±2.1)s、(14.3±1.7)s.B1已出现学习、记忆功能障碍,B3组更加加重,治疗组则明显减轻.(2)A1、B1、C1组海马部脑组织NSE蛋白表达量分别为0.081±0.009、0.292±0.034、0.090±0.011,A3、B3、C3组分别为0.089±0.013、0.402±0.068、0.184±0.028.B1、B3组较对照组明显增加,C1、C3组则明显减弱.S-100B变化与NSE结果有相似趋势.(3) A1、B1、C1组海马部脑组织NSE mRNA相对表达量分别为0.121±0.011、0.532±0.047、0.130±0.019,A3、B3、C3组分别为0.135±0.024、1.178±0.128、0.371±0.039.B1、B3组较对照组明显增加,C1、C3组则明显减弱.S-100B变化与NSE结果有相似趋势.(4)NSE和S-100B表达与水迷宫行为学测试成绩均呈显著正相关. 结论海马区NSE和S-100B表达升高是糖尿病脑病发病的重要因素,神经节苷脂可能通过降低NSE和S-100B的表达从而改善糖尿病脑病的认知功能障碍.
목적 평개신경절감지대당뇨병뇌병대서모형인지공능적작용,병탐토기영향궤제. 방법 60지대서안수궤수자표법분위정상대조조、당뇨병뇌병조화당뇨병뇌병+신경절감지치료조3조,매조재근거관찰시간점불동분위1월、3월량아조(A1、B1、C1、A3、B3、C3조),매아조각10지.채용련뇨좌균소건립대서당뇨병뇌병모형,당뇨병뇌병+신경절감지치료조매천복강주사신경절감지10 mg/kg.응용수미궁행위학측시관찰각조대서인지공능개변,면역조화염색、RT-PCR검측해마구신경원특이성희순화매(NSE)화S-100B단백급mRNA적표체. 결과 (1)A1、B1、C1조수미궁행위학측시성적분별위(6.7±0.5)s、(14.1±1.7)s、(6.9±0.6)s,A3、B3、C3조측시성적분별위(7.0±0.5)s、(26.6±2.1)s、(14.3±1.7)s.B1이출현학습、기억공능장애,B3조경가가중,치료조칙명현감경.(2)A1、B1、C1조해마부뇌조직NSE단백표체량분별위0.081±0.009、0.292±0.034、0.090±0.011,A3、B3、C3조분별위0.089±0.013、0.402±0.068、0.184±0.028.B1、B3조교대조조명현증가,C1、C3조칙명현감약.S-100B변화여NSE결과유상사추세.(3) A1、B1、C1조해마부뇌조직NSE mRNA상대표체량분별위0.121±0.011、0.532±0.047、0.130±0.019,A3、B3、C3조분별위0.135±0.024、1.178±0.128、0.371±0.039.B1、B3조교대조조명현증가,C1、C3조칙명현감약.S-100B변화여NSE결과유상사추세.(4)NSE화S-100B표체여수미궁행위학측시성적균정현저정상관. 결론 해마구NSE화S-100B표체승고시당뇨병뇌병발병적중요인소,신경절감지가능통과강저NSE화S-100B적표체종이개선당뇨병뇌병적인지공능장애.
Objective To evaluate the effect of ganglioside on cognitive function in rats with diabetic encephalopathy and investigate its mechanism.Methods Sixty Wistar rats were equally randomized into control group (A),diabetic encephalopathy group (B) and diabetic encephalopathy+ganglioside treatment group (C); each group was further divided into two subgroups according to the time points of 1 and 3 months (n=10).Ganglioside was injected intraperitoneally at 10 mg/kg daily in rats of the C1 and C3 groups.The cognitive function was investigated by Morris water maze test.Immunohistochemistry and RT-PCR were performed to determine the protein and mRNA expressions of neuron-specific enolase (NSE) and S-100B.Results (1) By Morris water maze test,the performances of A1,B1 and C1 groups were (6.7±0.5) s,(14.1±1.7) s and (6.9±0.6) s,while those of A3,B3 and C3 groups were (7.0±0.5) s,(26.6±2.1) s and (14.3±1.7) s.Mild dysfunction of learning and memory was detected in B1 group,and the dysfunction became worse in B3 group; while it was greatly improved in ganglioside-treatment groups.(2) Immunohistochemistry showed that the NSE protein expressions of A1,B1 and C1 groups were (0.081±0.009),(0.292±0.034) and (0.090±0.011),while those of A3,B3 and C3 groups were (0.089±0.013),(0.402±0.068) and (0.184±0.028); as compared with control groups,significant increases were noted in B1 and B3 groups,and dramatic reduces were noted in C1 and C3 groups; the tendency of S-100B protein expression was similar with NSE.(3) By RT-PCR,the NSE mRNA expressions of A1,B1 and C1 groups were (0.121±0.011),(0.532±0.047) and (0.130±0.019),while those of A3,B3 and C3 groups were (0.135±0.024),(1.178±0.128) and (0.371±0.039); as compared with control groups,significant increases were noted in B1 and B3 groups,and dramatic reduces were observed in C1 and C3 groups; the tendency of S-100B protein expression was similar with NSE.(4) Similarly,both NSE and S-100B expressions were positively correlated with Morris water maze test results.Conclusion Diabetic encephalopathy may be closely related to the hippocampal NSE and S-100B super-expressions; ganglioside has a protective effect by inhibiting NSE and S-100B expressions.