CAJ | 학술논문

目的观察胶质细胞相关受体甘氨酸转运体1(GlyT1)及胶质细胞源性神经营养因子(GDNF)在延髓面神经后核内侧区(mNRF)的表达情况.方法新生SD大鼠(0～3 d) 10只,雌雄不拘,按随机数字表法分成对照组、实验组(n=5).取实验组大鼠离体延髓-脊髓标本制作mNRF“岛”,对照组大鼠取三叉神经核周围脑组织,荧光定量RT-PCR测定GlyT1、GDNF在mNRF“岛”和三叉神经核周围脑组织的表达.结果实验组mNRF“岛”区GlyT1、GDNF基因的表达量均高于对照组三叉神经核周围脑组织中的基因表达量,差异有统计学意义(t=5.112,P=0.000; t=3.532,P=0.003).结论 GlyT1、GDNF在mNRF存在高表达,但这两种物质在节律性呼吸发生及调控过程中的作用及与神经元间的具体联系尚有待进一步研究.
목적 관찰효질세포상관수체감안산전운체1(GlyT1)급효질세포원성신경영양인자(GDNF)재연수면신경후핵내측구(mNRF)적표체정황.방법 신생SD대서(0～3 d) 10지,자웅불구,안수궤수자표법분성대조조、실험조(n=5).취실험조대서리체연수-척수표본제작mNRF“도”,대조조대서취삼차신경핵주위뇌조직,형광정량RT-PCR측정GlyT1、GDNF재mNRF“도”화삼차신경핵주위뇌조직적표체.결과 실험조mNRF“도”구GlyT1、GDNF기인적표체량균고우대조조삼차신경핵주위뇌조직중적기인표체량,차이유통계학의의(t=5.112,P=0.000; t=3.532,P=0.003).결론 GlyT1、GDNF재mNRF존재고표체,단저량충물질재절률성호흡발생급조공과정중적작용급여신경원간적구체련계상유대진일보연구.
Objective To investigate the mRNA expressions of glial glycine transporter 1 (GlyT1) and glial cell line-derived neurotrophic factor (GDNF) genes in the medial area of nucleus retrofacialis (mNRF).Methods Ten neonatal SD rats (aged 0-3 d) were randomly divided into control and experimental groups (n=5); the mNRF "islands" were isolated from the brainstem slices in rats of the experimental group,while trigeminal nucleus surrounding brain tissues were chosen in rats of the control group.GlyT1 and GDNF gene expressions were detected using real time quantitative PCR (RT-PCR).Results The expressions of GlyT1 and GDNF genes in the mNRF "island" were both significantly higher than those in the controls (t=5.112,P=0.000; t=3.532,P=0.003).Conclusion ThemRNA expressions of GlyT1 and GDNF genes are high in the mNRF; however,the roles of them in generation and modulation in rhythmical respiration and the mechanisms between neurons and both GlyT1 and GDNF should be further studied.