CAJ | 학술논문

目的探讨HAUSP、NANOG在胶质瘤细胞中的定位及在人脑胶质瘤组织中的表达,了解二者在胶质瘤进展中的作用. 方法取安徽医科大学附属省立医院神经外科自2010年1月至2010年11月间手术切除的胶质瘤标本72例,其中WHOⅡ级19例、WHOⅢ级25例、WHOⅣ级28例,采用免疫组织化学染色检测HAUSP、NANOG蛋白在胶质瘤中的表达;常规培养U87胶质瘤细胞株,双重免疫荧光细胞化学染色检测U87细胞HAUSP/NANOG的亚细胞定位.结果不同级别胶质瘤中HAUSP蛋白阳性细胞率比较差异有统计学意义(F=15.926,P=0.000),且胶质瘤的病理级别越高,HAUSP蛋白阳性细胞率越高,差异有统计学意义(P＜0.05)；胶质瘤的病理级别和HAUSP蛋白表达水平呈正相关关系(r=0.726,P=0.000)；不同级别胶质瘤中NANOG蛋白阳性细胞率比较差异有统计学意义(F=14.768,P=-0.000),且胶质瘤的病理级别越高,NANOG蛋白阳性细胞率越高,差异有统计学意义(P＜0.05)；胶质瘤的病理级别和NANOG蛋白表达水平呈正相关关系(r=0.685,P=0.000)；胶质瘤细胞U87中,两者均有表达,且两者共定位于细胞核. 结论 HAUSP与NANOG两者在胶质瘤发病中起重要作用,且与肿瘤恶性度相关;NANOG作为维持胶质瘤细胞未分化或低分化状态的标记物,HAUSP的去泛素化作用可能对于NANOG维持胶质瘤细胞的未分化状态有重要作用.
목적 탐토HAUSP、NANOG재효질류세포중적정위급재인뇌효질류조직중적표체,료해이자재효질류진전중적작용. 방법 취안휘의과대학부속성립의원신경외과자2010년1월지2010년11월간수술절제적효질류표본72례,기중WHOⅡ급19례、WHOⅢ급25례、WHOⅣ급28례,채용면역조직화학염색검측HAUSP、NANOG단백재효질류중적표체;상규배양U87효질류세포주,쌍중면역형광세포화학염색검측U87세포HAUSP/NANOG적아세포정위.결과 불동급별효질류중HAUSP단백양성세포솔비교차이유통계학의의(F=15.926,P=0.000),차효질류적병리급별월고,HAUSP단백양성세포솔월고,차이유통계학의의(P＜0.05)；효질류적병리급별화HAUSP단백표체수평정정상관관계(r=0.726,P=0.000)；불동급별효질류중NANOG단백양성세포솔비교차이유통계학의의(F=14.768,P=-0.000),차효질류적병리급별월고,NANOG단백양성세포솔월고,차이유통계학의의(P＜0.05)；효질류적병리급별화NANOG단백표체수평정정상관관계(r=0.685,P=0.000)；효질류세포U87중,량자균유표체,차량자공정위우세포핵. 결론 HAUSP여NANOG량자재효질류발병중기중요작용,차여종류악성도상관;NANOG작위유지효질류세포미분화혹저분화상태적표기물,HAUSP적거범소화작용가능대우NANOG유지효질류세포적미분화상태유중요작용.
Objective To study the subcellular location of HAUSP and NANOG in glioma cells and their expressions in glioma tissues,and explore their significance in the pathogenesis of gliomas.Methods Seventy-two glioma samples (19 with WHO graded Ⅱ,25 with WHO graded Ⅲ and 28 with WHO graded Ⅳ),collected in our hospital from January 2010 to November 2010,were chosen in our study; protein expressions of HAUSP and NANOG in the glioma tissues were detected by immunohistochemical staining; the subcellular location of HAUSP and NANOG in human glioma cell line U87 was detected by double immunofluorescence staining.Results The positive rate of HAUSP protein in gliomas with different grades was significantly different (F=15.926,P=0.000); the higher the malignancy grade,the higher the positive rate of HAUSP protein (P＜0.05); the malignancy grade of gliomas was positively correlated with the protein expression of HAUSP (r=0.726,P=0.000).The positive rate of NANOG protein in gliomas with different grades was significantly different (F=14.768,P=0.000);the higher the malignancy grade,the higher the positive rate of NANOG protein (P＜0.05); the malignancy grade ofgliomas was positively correlated with the protein expression of NANOG (r=0.685,P=0.000).Both NANOG and HAUSP expressed in U87,and co-located in the nucleus.Conclusion HAUSP and NANOG play important roles in the pathogenesis of human gliomas and are related to the malignancy; NANOG,as the marker of glioma stem cells in undifferentiated state,plays a vital role in maintaining the cancer stem cells in undifferentiated state through deubiquitinylation of HAUSP.