CAJ | 학술논문

目的通过CatWalk步态分析系统评价离断L5脊神经根对下肢功能的影响,以明确将其用于构建膀胱反射弧的可行性. 方法选择SD雄性大鼠11只,以大鼠右侧为手术侧,将其L5脊神经前、后根截断,左侧不做处理作为对照侧.术后1d3只大鼠行麦芽凝集素辣根过氧化物酶(WGA-HRP)神经示踪实验验证模型是否构建成功,应用CatWalk步态分析系统测试大鼠脊神经离断术前1d及术后3月一般性参数、个体化肢体参数、疼痛相关性参数、协调相关性参数的变化.结果神经示踪实验结果显示实验侧L5节段脊髓前、后角均未见阳性神经元及神经纤维,对照侧L5节段脊髓前角、后角均可见阳性神经元及神经纤维,从而证实模型构建成功；术后3月大鼠的一般性参数、个体化肢体参数、疼痛相关性参数等与术前1d比较差异无统计学意义(P＜0.05),但协调相关性参数中规律性参数(RJ)及右后-左前联动与术前1d相比下降,差异有统计学意义(P＜0.05). 结论离断L5脊神经后仅影响下肢行走的协调性,对其余步态相关参数无影响,因而可用于膀胱反射弧的构建.
목적 통과CatWalk보태분석계통평개리단L5척신경근대하지공능적영향,이명학장기용우구건방광반사호적가행성. 방법 선택SD웅성대서11지,이대서우측위수술측,장기L5척신경전、후근절단,좌측불주처리작위대조측.술후1d3지대서행맥아응집소랄근과양화물매(WGA-HRP)신경시종실험험증모형시부구건성공,응용CatWalk보태분석계통측시대서척신경리단술전1d급술후3월일반성삼수、개체화지체삼수、동통상관성삼수、협조상관성삼수적변화.결과 신경시종실험결과현시실험측L5절단척수전、후각균미견양성신경원급신경섬유,대조측L5절단척수전각、후각균가견양성신경원급신경섬유,종이증실모형구건성공；술후3월대서적일반성삼수、개체화지체삼수、동통상관성삼수등여술전1d비교차이무통계학의의(P＜0.05),단협조상관성삼수중규률성삼수(RJ)급우후-좌전련동여술전1d상비하강,차이유통계학의의(P＜0.05). 결론 리단L5척신경후부영향하지행주적협조성,대기여보태상관삼수무영향,인이가용우방광반사호적구건.
Objective To assess the functional changes of the hind paw after SD rats being cut offthe L5 spinal nerve by CatWalk gait analysis.Methods Eleven male SD rats were chosen; the right side of these rats,as the experimental side,was cut the ventral root and dorsal root of L5 spinal nerve,while the left side was without any operation.One day after the operation,whether the models were successfully constructed was verified by tracer experiment with wheat germ agglutinin horseradish peroxidase (WGA-HRP) in 3 rats; 1 day before the operation and 3 months after the operation,the changes of such parameters as general parameters,individual paw parameters,pain-related parameters and coordination-related parameters were assessed by using CatWalk gait analysis.Results The WGA-HRP-labeled motoneurons and fibers were not found in posterior horn and anterior horn of the experimental side of L5 spinal gray matter,but both located in the left side,indicating that the models were constructed successfully.General parameters,individual paw parameters and pain-related gait parameters could be recorded 3 months after the operation,but no statistically significant difference was noted as compared with those 1 day before the operation; however,significant decrease of values of regularity index (RI) and coupling diagonal right after-left front in rats 3 months after the operation was noted as compared with those 1 day before the operation (P＜0.05).Conclusion Only a part of coordination-related gait parameters (the lower limbs) is influenced after being cut L5 spinal nerve;therefore,L5 spinal nerve can be used for constructing of a bladder reflex arc.