CAJ | 학술논문

目的探讨岛叶在杏仁核点燃大鼠癫痫发作中的作用. 方法健康雄性SD大鼠48只,按照随机数字表法分为空白组(n=8)、假手术组(n=8)和点燃组(n=32),点燃组以最后一次刺激为零点,再分为点燃后1h、3h、6h、12h4个亚组,每亚组8只.空白组不做任何处理；假手术组植入电极后不予刺激；点燃组植入电极后予以电刺激,制作杏仁核点燃模型.采用荧光原位杂交和免疫组化检测大鼠海马、岛叶脑区活性调节细胞骨架蛋白(Arc) mRNA和蛋白表达的变化. 结果点燃大鼠海马、岛叶的Arc mRNA在点燃后1h开始增高,3h达到高峰,与空白组、假手术组比较,差异均有统计学意义(P＜0.05);6 h时回到正常水平.Arc蛋白在点燃后3h开始增高,6h达到高峰,与空白组、假手术组比较,差异均有统计学意义(P＜0.05)；12h时回到正常水平.假手术组与空白组之间Arc mRNA和蛋白差异没有统计学意义(P＞0.05). 结论岛叶与杏仁核、海马构成了一个灶性复合体,共同参与了颞叶癫痫的发生.
목적 탐토도협재행인핵점연대서전간발작중적작용. 방법 건강웅성SD대서48지,안조수궤수자표법분위공백조(n=8)、가수술조(n=8)화점연조(n=32),점연조이최후일차자격위영점,재분위점연후1h、3h、6h、12h4개아조,매아조8지.공백조불주임하처리；가수술조식입전겁후불여자격；점연조식입전겁후여이전자격,제작행인핵점연모형.채용형광원위잡교화면역조화검측대서해마、도협뇌구활성조절세포골가단백(Arc) mRNA화단백표체적변화. 결과 점연대서해마、도협적Arc mRNA재점연후1h개시증고,3h체도고봉,여공백조、가수술조비교,차이균유통계학의의(P＜0.05);6 h시회도정상수평.Arc단백재점연후3h개시증고,6h체도고봉,여공백조、가수술조비교,차이균유통계학의의(P＜0.05)；12h시회도정상수평.가수술조여공백조지간Arc mRNA화단백차이몰유통계학의의(P＞0.05). 결론 도협여행인핵、해마구성료일개조성복합체,공동삼여료섭협전간적발생.
Objective To investigate the role of insular cortex in amygdala-kindled seizures in rats.Methods Forty-eight healthy male SD rats were randomly divided into blank control group (n=8),sham-operated group (n=8) and amygdala-kindled group (n=32); no treatment was performed in the blank control group,and only implantation of electrodes was performed in the sham-operated group; implantation of electrodes and electrophotoluminescence were performed in the amygdala-kindled group to induce amygdala-kindled seizure models.Rats in the amygdala-kindled group was divided into 4sub-groups (n=8) at different times after the kindling (1,3,6 and 12 h).Fluorescence in situ hybridization and immunohistochemistry staining were employed to investigate the altered mRNA and protein expressions of activity-regulated cytoskeleton-associated protein (Arc) in the hippocampus and insula of the rat brain.Results As compared with that in the blank control group and sham-operated group,Arc mRNA expression in the amygdala-kindled sub-groups increased at 1 h after the kindling (P＜0.05),peaked at 3 h after the kindling (P＜0.05),and returned to basic level at 6 h after the kindling (P＞0.05).As compared with that in the blank control group and sham-operated group,Arc protein expression in the amygdala-kindled sub-groups increased at 3 h after the kindling,(P＜0.05),peaked at 6 h after the kindling (P＜0.05),and returned to basic level at 12 h after the kindling (P＞0.05).No significant difference on mRNA and protein expressions of Arc was noted between the sham-operated group and blank control group (P＞0.05).Conclusion Insular cortex,the amygdala and the hippocampus form a focus complex,which participates in the occurrence of temporal lobe epilepsy.