CAJ | 학술논문

目的探讨星形细胞瘤组织中特异性的核基质结合区结合蛋白-1 (SA TB1)基因、SLC22A18蛋白的表达、与肿瘤恶性程度及两者之间的相互关系. 方法选取上海交通大学医学院附属第三人民医院神经外科自2006年9月至2010年6月、武汉大学中南医院神经外科自2003年9月至2006年6月间手术切除的星形细胞瘤标本56例,其中WHO分级Ⅰ级12例,Ⅱ级13例,Ⅲ级15例,Ⅳ级16例.另选10例行内减压术颅脑损伤患者的正常脑组织作为对照组.应用RT-PCR和Western blotting分别检测各标本中SATB1mRNA和蛋白的表达,免疫组织化学法检测各标本中SLC22A18蛋白的表达,分析二者与肿瘤恶性程度及两者之间的相互关系. 结果 RT-PCR与Western blotting检测显示对照组脑组织中SATB1mRNA和蛋白有少许表达,星形细胞瘤中35例表达阳性；不同级别星形细胞瘤SATB1mRNA和蛋白的阳性表达率、表达值不同,且随着肿瘤病理级别的增高,SATB1mRNA和蛋白的表达值增强,差异有统计学意义(P＜0.05).SATB1蛋白的表达值与肿瘤的病理级别呈正相关关系(r=0.987,P=0.000);免疫组织化学法检测显示对照组均检测到SLC22A18表达,星形细胞瘤中19例SLC22A18表达阳性.不同级别星形细胞瘤SLC22A18的阳性表达率不同,随着肿瘤病理级别的增高,SLC22A18的阳性表达率降低,差异有统计学意义(P＜0.05); SLC22A18表达阴性组中SATB1蛋白阳性表达率(81.1％)显著高于SLC22A18表达阳性组(263％),差异有统计学意义(P＜0.05). 结论 SATB1基因在星形细胞瘤中表达,提示该基因对星形细胞瘤发生和发展起重要作用；抑癌基因SLC22A18的失活与SATB1基因的表达可能在星形细胞瘤的发生中起协同作用.
목적 탐토성형세포류조직중특이성적핵기질결합구결합단백-1 (SA TB1)기인、SLC22A18단백적표체、여종류악성정도급량자지간적상호관계. 방법 선취상해교통대학의학원부속제삼인민의원신경외과자2006년9월지2010년6월、무한대학중남의원신경외과자2003년9월지2006년6월간수술절제적성형세포류표본56례,기중WHO분급Ⅰ급12례,Ⅱ급13례,Ⅲ급15례,Ⅳ급16례.령선10례행내감압술로뇌손상환자적정상뇌조직작위대조조.응용RT-PCR화Western blotting분별검측각표본중SATB1mRNA화단백적표체,면역조직화학법검측각표본중SLC22A18단백적표체,분석이자여종류악성정도급량자지간적상호관계. 결과 RT-PCR여Western blotting검측현시대조조뇌조직중SATB1mRNA화단백유소허표체,성형세포류중35례표체양성；불동급별성형세포류SATB1mRNA화단백적양성표체솔、표체치불동,차수착종류병리급별적증고,SATB1mRNA화단백적표체치증강,차이유통계학의의(P＜0.05).SATB1단백적표체치여종류적병리급별정정상관관계(r=0.987,P=0.000);면역조직화학법검측현시대조조균검측도SLC22A18표체,성형세포류중19례SLC22A18표체양성.불동급별성형세포류SLC22A18적양성표체솔불동,수착종류병리급별적증고,SLC22A18적양성표체솔강저,차이유통계학의의(P＜0.05); SLC22A18표체음성조중SATB1단백양성표체솔(81.1％)현저고우SLC22A18표체양성조(263％),차이유통계학의의(P＜0.05). 결론 SATB1기인재성형세포류중표체,제시해기인대성형세포류발생화발전기중요작용；억암기인SLC22A18적실활여SATB1기인적표체가능재성형세포류적발생중기협동작용.
Objective To study the expression of special AT-rich sequence-binding protein 1(SATB1) gene and its relationship with SLC22A18 protein expression in astrocytoma.Methods Fifty-six patients with astrocytomas (12 with grade Ⅰ,13 with grade Ⅱ,15 with grade Ⅲ,and 16 with grade Ⅳ),performed surgical excision in our hospitals from September 2006 to June 2010 and from September 2003 to June 2006,were chosen in our study; another 10 brain tissues from patients performed decompression operation resulting from cerebral hernia were selected as the controls.RT-PCR and Western blotting were used to detect the mRNA and protein expressions of SATB1.The SLC22A18 protein expression was detected by immunohistochemical assay.The relations between SLC22A18expressions and SA TB1 levels,and these two and the degree of malignancy were analyzed.Results RT-PCR and Western blotting revealed that positive mRNA and protein expressions were noted in 35patients with astrocytomas; the mRNA and protein expression rate and value of SATB1 in the astrocytoma tissues were significantly different among different grades of tumors (P＜0.05); the higher the malignancy grade,the higher mRNA and protein expression rate and value ofSA TB1; the protein expression value of SA TB1 had a positive correlation with the malignancy grade of tumors (r=0.987,P=0.000).And a few expressions of SA TB1 mRNA and protein were found in the tissues of controls.Immunohistochemical assay indicated that positive protein expression of SLC22A18 was noted in 19 astrocytoma tissues,and the protein expression rate of SLC22A18 in the astrocytoma tissues was significantly different among different grades of tumors (P＜0.05); the higher the malignancy grade,the lower expression of SLC22A18.And the protein expression of SLC22A18 was found in all the tissues of controls.The SATB1 expression rate in the tissues with negative SLC22A18 expression (81.1％) was significantly higher than that in the tissues with positive SLC22A18 expression (26.3％,P＜0.05).Conclusion SATB1 expresses in the astrocytoma tissues,indicating that it may play an important role in the pathogenesis of astrocytoma;up-regulation of SATB1 expression and dysfunction of SLC22A18 may play synergetic roles in the process of carcinogenesis of astrocytoma.