CAJ | 학술논문

目的研究人胎盘底蜕膜间充质干细胞(hPDB-MSCs)抗炎特性对帕金森病(PD)模型大鼠多巴胺能神经元的影响. 方法体外培养hPDB-MSCs,6-羟基多巴(6-OHDA)制备大鼠PD模型并按照随机数字表法分为模型组与移植组,每组32只.hPDB-MSCs尾静脉移植观察各组大鼠行为学改变.免疫组化检测移植后3d、1周、2周及4周各组大鼠损伤侧黑质酪氨酸羟化酶(TH)、离子钙接头蛋白(Ibal)表达.实时荧光定量PCR检测各时间点各组大鼠损伤侧黑质抗炎因子人白细胞介素-10 (hIL-10)、人转化生长因子-β(hTGF-β)及肿瘤坏死因子-α(TNF-α)表达. 结果 hPDB-MSCs移植后1周、2周、4周,移植组大鼠阿朴吗啡诱导的平均旋转圈数明显低于模型组,差异有统计学意义(P＜0.05).免疫组化结果显示移植组大鼠损伤侧黑质部位TH阳性细胞数较模型组明显增加,1周、2周、4周时差异有统计学意义(P＜0.05).移植组大鼠损伤侧黑质部位Ibal阳性细胞则明显减少,4周时最为显著.mRNA水平,移植组大鼠hIL-10及hTGF-β表达均较模型组增加,而TNF-α表达量则逐渐降低. 结论 hPDB-MSCs尾静脉移植后能够通过抗炎机制抑制PD模型大鼠多巴胺能神经元丧失,改善PD模型大鼠症状.
목적 연구인태반저세막간충질간세포(hPDB-MSCs)항염특성대파금삼병(PD)모형대서다파알능신경원적영향. 방법 체외배양hPDB-MSCs,6-간기다파(6-OHDA)제비대서PD모형병안조수궤수자표법분위모형조여이식조,매조32지.hPDB-MSCs미정맥이식관찰각조대서행위학개변.면역조화검측이식후3d、1주、2주급4주각조대서손상측흑질락안산간화매(TH)、리자개접두단백(Ibal)표체.실시형광정량PCR검측각시간점각조대서손상측흑질항염인자인백세포개소-10 (hIL-10)、인전화생장인자-β(hTGF-β)급종류배사인자-α(TNF-α)표체. 결과 hPDB-MSCs이식후1주、2주、4주,이식조대서아박마배유도적평균선전권수명현저우모형조,차이유통계학의의(P＜0.05).면역조화결과현시이식조대서손상측흑질부위TH양성세포수교모형조명현증가,1주、2주、4주시차이유통계학의의(P＜0.05).이식조대서손상측흑질부위Ibal양성세포칙명현감소,4주시최위현저.mRNA수평,이식조대서hIL-10급hTGF-β표체균교모형조증가,이TNF-α표체량칙축점강저. 결론 hPDB-MSCs미정맥이식후능구통과항염궤제억제PD모형대서다파알능신경원상실,개선PD모형대서증상.
Objective To investigate whether human placental decidua basalis-derived mesenchymal stem cells (hPDB-MSCs) by tail vein infusion can inhibit the loss of dopaminergic neuron in rat models of Parkinson's disease (PD) via an anti-inflammatory mechanism.Methods The hPDB-MSCs were cultured in vitro; PD rat models were established by intracranial injection of 6-hydroxydopamine (6-OHDA),and then,they were divided into vehicle group and transplantation group; hPDB-MSCs were infused into the rats of transplantation group by tail vein.Behavior changes of each group were evaluated by apomorphine (APO)-induced rotational test.Substantia nigra (SN) of the injured side in each group 3 days and 1,2 and 4 weeks after transplantation were stained with immunohistochemistry to observe the expression changes of tyrosine hydroxylase (TH) and ionized calcium bindingadaptor molecule-1 (Ibal).The expressions of human interleukin-10 (hIL-10),human transforming growth factor-β (hTGF-β) and tumor necrosis factor-α (TNF-α) in the substantia nigra (SN) of the injured side in each group 3 days and 1,2 and 4 weeks after transplantation were detected by real time PCR.Results The number of mean APO-induced rotational circle in the transplantation group at 1,2 and 4 weeks after transplantation was significantly smaller than that in the vehicle group (P＜0.05).Significant increase of TH-positive cells in the lesioned side of SN 4 weeks after transplantation was noted as compared with that of SN 3 day after transplantation in the transplantation group (P＜0.05);significant increase of TH-positive cells in the lesioned side of SN was also noted between the two groups 2 and 4 weeks after transplantation (P＜0.05); the number of actived microglias in the lesioned side of SN decreased in the transplanted group 4 weeks after transplantation.The mRNA expression levels ofhlL-10 and hTGF-β in the transplantation group 1 and 2 weeks after the transplantation were significantly higher than those in the vehicle group,while the mRNA expression level of TNF-α was lower than that in vehicle group.Conclusion The hPDB-MSCs by tail vein infusion can inhibit the loss ofdopaminergic neuron via an anti-inflammatory mechanism to improve the symptoms of the PD rats.