CAJ | 학술논문

目的探讨银丹心脑通软胶囊对血管性痴呆(VD)大鼠学习记忆能力及大脑皮层反应性氮中介物含量的影响. 方法将SD大鼠按随机数字表法分为假手术组、VD组、银丹心脑通软胶囊治疗组,后两组采用永久性结扎双侧颈总动脉方法制备VD模型,银丹心脑通软胶囊治疗组于手术后即刻给予银丹心脑通软胶囊60 mg/(kg·d)灌胃,连续给药4周.4周后采用Morris水迷宫试验观察各组大鼠学习记忆能力的改变,采用比色法检测大鼠大脑皮层一氧化氮(NO)、诱导型一氧化氮合酶(iNOS)含量的变化. 结果 VD组与银丹心脑通软胶囊治疗组、假手术组比较,逃避潜伏期明显延长,原平台所在象限的搜索时间明显缩短,差异有统计学意义(P＜0.05).银丹心脑通软胶囊治疗组较VD组逃避潜伏期明显缩短、原平台所在象限的搜索时间明显延长,差异有统计学意义(P＜0.05).银丹心脑通软胶囊治疗组与VD组相比,大脑皮层NO和iNOS含量明显下降,差异有统计学意义(P＜0.05). 结论银丹心脑通软胶囊能降低VD大鼠皮层NO、iNOS含量,对学习记忆能力有明显的提高作用.
목적 탐토은단심뇌통연효낭대혈관성치태(VD)대서학습기억능력급대뇌피층반응성담중개물함량적영향. 방법 장SD대서안수궤수자표법분위가수술조、VD조、은단심뇌통연효낭치료조,후량조채용영구성결찰쌍측경총동맥방법제비VD모형,은단심뇌통연효낭치료조우수술후즉각급여은단심뇌통연효낭60 mg/(kg·d)관위,련속급약4주.4주후채용Morris수미궁시험관찰각조대서학습기억능력적개변,채용비색법검측대서대뇌피층일양화담(NO)、유도형일양화담합매(iNOS)함량적변화. 결과 VD조여은단심뇌통연효낭치료조、가수술조비교,도피잠복기명현연장,원평태소재상한적수색시간명현축단,차이유통계학의의(P＜0.05).은단심뇌통연효낭치료조교VD조도피잠복기명현축단、원평태소재상한적수색시간명현연장,차이유통계학의의(P＜0.05).은단심뇌통연효낭치료조여VD조상비,대뇌피층NO화iNOS함량명현하강,차이유통계학의의(P＜0.05). 결론 은단심뇌통연효낭능강저VD대서피층NO、iNOS함량,대학습기억능력유명현적제고작용.
Objective To explore the effect of Yindan Xinnaotong capsules (YDXNT) on the reactive nitrogen intermediates in the cerebral cortex and learning and memory abilities of rats with cerebral ischemia.Methods Thirty rats were randomly divided into sham-operated group,vehicle group and Yindan Xinnaotong capsule treatment group.The animal models of cerebral ischemia in the later two groups were established by two vessel occlusion.Yindan Xinnaotong capsule 60 mg/(kg·d) via inttragastric administration was given to the Yindan Xinnaotong capsule treatment group for a consecutive four weeks.The learning and memory ability changes were tested with Morris water maze and the contents of nitric oxide (NO) and inducible nitric-oxide synthase (iNOS) in the cerebral cortex were measured by colorimetric method.Results As compared with the vehicle group,the Yindan Xirnaotong capsule treatment group and sham-operated group had significantly shorter escape latency and longer searching time in the quadrant of primary platform (P＜0.05); As compared with the vehicle group,the Yindan Xinnaotong capsule treatment group had significant decreased contents of NO and iNOS in the cerebral cortex (P＜0.05).Conclusion Yindan Xinnaotong capsules can improve the learning and memory abilities of rats with cerebral ischemia by lowering the activity of NO and iNOS in the cerebral cortex.