CAJ | 학술논문

目的从脑肠轴角度明确肠易激综合征(IBS)发病机制,观察姜黄素对IBS的疗效.方法 48只SD大鼠按随机数字表法分为6组:正常组,IBS模型组,姜低组、姜中组、姜高组(造模前分别灌胃给予姜黄素10、20、40 mg/kg),丙咪嗪组(造模前腹腔注射丙咪嗪10 mg/kg),每组各8只.采用大鼠慢急性联合应激模型模拟IBS.糖水消耗试验和内脏敏感性实验检测各组大鼠抑郁样行为和肠道敏感性,高效液相法及Western blotting分别测定大鼠大脑皮层和回肠5-羟色胺(5-HT)及脑源性神经营养因子(BDNF)的表达. 结果 IBS模型组大鼠糖水消耗量明显减少,肠道内脏敏感性明显升高,大脑额叶皮层5-HT、BDNF表达水平降低,回肠黏膜5-HT、BDNF表达水平明显升高,与正常组比较差异均有统计学意义(P＜0.05).给予不同剂量姜黄素后能逆转上述行为学改变及大脑皮层、回肠的5-HT 、BDNF水平变化,其中姜高组与IBS模型组比较差异均有统计学意义(P＜0.05).丙咪嗪组效果与姜高组类似. 结论 IBS大鼠脑肠内5-HT、BDNF表达异常说明IBS的发生存在脑肠改变的物质基础.姜黄素可能是通过调节5-HT、BDNF表达来改善IBS模型大鼠的行为学变化.
목적 종뇌장축각도명학장역격종합정(IBS)발병궤제,관찰강황소대IBS적료효.방법 48지SD대서안수궤수자표법분위6조:정상조,IBS모형조,강저조、강중조、강고조(조모전분별관위급여강황소10、20、40 mg/kg),병미진조(조모전복강주사병미진10 mg/kg),매조각8지.채용대서만급성연합응격모형모의IBS.당수소모시험화내장민감성실험검측각조대서억욱양행위화장도민감성,고효액상법급Western blotting분별측정대서대뇌피층화회장5-간색알(5-HT)급뇌원성신경영양인자(BDNF)적표체. 결과 IBS모형조대서당수소모량명현감소,장도내장민감성명현승고,대뇌액협피층5-HT、BDNF표체수평강저,회장점막5-HT、BDNF표체수평명현승고,여정상조비교차이균유통계학의의(P＜0.05).급여불동제량강황소후능역전상술행위학개변급대뇌피층、회장적5-HT 、BDNF수평변화,기중강고조여IBS모형조비교차이균유통계학의의(P＜0.05).병미진조효과여강고조유사. 결론 IBS대서뇌장내5-HT、BDNF표체이상설명IBS적발생존재뇌장개변적물질기출.강황소가능시통과조절5-HT、BDNF표체래개선IBS모형대서적행위학변화.
Objective To investigate the pathogenesis of irritable bowel syndrome (IBS) from the aspect of brain-gut interaction and find out the efficacy and mechanism of curcumin on IBS.Methods Forty-eight rats were chosen in our study,and divided into 6 groups (n=8):normal group,IBS model group,IBS+curcumin treatment groups (10,20 and 40 mg/kg of curcumin via intragastric administration 1 h before model inducement) and IBS+imipramine treatment group (10 mg/kg,via intraperitoneal injection 30 min before model inducement); IBS rat models were established by chronic and acute stress; treatments in each group were given for a consecutive 21 days.The depression-like behaviors and gut hypersensitivity of the rats in all the groups were detected.The expressions of 5-hydroxytryptamine (5-HT) and brain-derived neurotrophic factor (BDNF) in the cerebral cortex and ileum were determined by high performance liquid chromatography (HPLC) and Western blotting,respectively.Results The sucrose consumption of IBS rat had been significantly reduced and the intestinal viscera sensitivity was obviously elevated in the IBS model group as compared with those in the normal group (P＜0.05); as compared with those in the normal control group,the expression of 5-HT significantly decreased inthe cerebral cortex of IBS modelgroup (P＜0.05),but opposite presentations were noted in the ileum (P＜0.05).As compared with that in the normal group,the BDNF expression in the brain frontal cortex was significantly decreased but obviously increased in the ileum (P＜0.05).High doses of curcumin (40 mg/kg treatment groups) improved the behavior ofIBS rats,and reversed the levels of 5-HT in the cerebral cortex and ileum,with significant difference (P＜0.05); besides,they could significantly reverse the BDNF level in the cerebral cortex and ileum,with significant difference (P＜0.05).Conclusion IBS rat models have abnormal expressions of 5-HT and 5-HIAA,and BDNF in the brain-gut system,which indicates that alterations of brain-gut exist during IBS; curcumin can improve the behavior changes and regulate the expression of serotonin and BDNF in IBS rats.