CAJ | 학술논문

目的探讨脑损伤后脑脊液中基质金属蛋白酶-9(MMP-9)含量变化及其临床意义.方法临床收集深圳市宝安区人民医院神经外科自2010年1月至2012年6月收治的69例不同类型脑损伤患者脑脊液标本为实验组,另外选取35例健康者脑脊液标本为对照组.采用ELISA法检测实验组24 h、72 h、1周及2周时脑脊液中MMP-9的含量变化,并与对照组进行比较分析.结果测试发现实验组MMP-9在脑脊液中的活性明显升高,可能与脑损伤程度、脑水肿发生以及脑损伤后疾病的转归有密切关系.实验组所有脑脊液标本中MMP-9含量显著高于对照组,差异有统计学意义(P＜0.05). 结论脑损伤时脑脊液中MMP-9的含量有明显改变,在急性期可能参与脑水肿病理过程,影响脑损伤临床治疗和转归.
목적 탐토뇌손상후뇌척액중기질금속단백매-9(MMP-9)함량변화급기림상의의.방법 림상수집심수시보안구인민의원신경외과자2010년1월지2012년6월수치적69례불동류형뇌손상환자뇌척액표본위실험조,령외선취35례건강자뇌척액표본위대조조.채용ELISA법검측실험조24 h、72 h、1주급2주시뇌척액중MMP-9적함량변화,병여대조조진행비교분석.결과 측시발현실험조MMP-9재뇌척액중적활성명현승고,가능여뇌손상정도、뇌수종발생이급뇌손상후질병적전귀유밀절관계.실험조소유뇌척액표본중MMP-9함량현저고우대조조,차이유통계학의의(P＜0.05). 결론 뇌손상시뇌척액중MMP-9적함량유명현개변,재급성기가능삼여뇌수종병리과정,영향뇌손상림상치료화전귀.
Objective To investigate the changes of matrix Metalloproteinases-9 (MMP-9) content in the cerebrospinal fluid of patients with brain injury and its clinical significance.Methods Sixty cerebrospinal fluid (CSF) specimens from patients with traumatic brain injury were chosen in our study as experimental group,and other CSF samples from 35 healthy subjects were selected as control group.The content of MMP-9 in cerebrospinal fluid was measured by enzyme-linked immunosorbent assay (ELISA) at 24 h and 72 h,1 and 2 weeks,respectively; and the comparative study was performed.Results The MMP-9 content in all CSF samples from the experimental group was significantly higher than that in the healthy control group (P＜0.05).Conclusion MMP-9 content in CSF of patients with brain injury increases obviously; MMP-9 in CSF may contribute to the severity of traumatic brain injury determination and guide the treatment strategies,which is worth for further study.