中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2013年
12期
1234-1238
,共5页
邓跃飞%李忠军%庞家栋%张黎明
鄧躍飛%李忠軍%龐傢棟%張黎明
산약비%리충군%방가동%장려명
神经胶质瘤%氧化石墨烯%转铁蛋白%纳米化%荧光显像
神經膠質瘤%氧化石墨烯%轉鐵蛋白%納米化%熒光顯像
신경효질류%양화석묵희%전철단백%납미화%형광현상
Glioma%Graphene oxide%Transferrin%Nanocrystallization%Fluorescence imaging
目的 制备功能化纳米氧化石墨烯(nano-GO-Tf-FITC)微粒,并研究其在近红外线(NIR)照射下对脑胶质瘤U251细胞的靶向荧光显像作用. 方法 将氧化石墨烯(GO)超声振荡制得纳米单层GO(nano-GO)微粒,以聚赖氨酸叠氮基团(poly-L-lysine-G3)连接靶向分子转铁蛋白(Tf)和荧光分子异硫氰酸荧光素(FITC),制备出功能化nano-GO-Tf-FITC微粒,应用透射电镜在一定倍数下观察并拍摄样品形貌,测定样品粒径.将培养的脑胶质瘤U251细胞分成3组,即靶向实验组(加入nano-GO-Tf-FITC微粒孵育)、平行对照组(加入抗CD71-FITC试剂孵育)和非靶向对照组(加入nano-GO-FITC微粒孵育),采用荧光显微镜检测其荧光显像. 结果 在高分辨率透射电镜下nano-GO为20～100 nm单片层纳米颗粒,功能化nano-GO-Tf-FIT微粒为分散在＜100 nm的单片层.在荧光显微镜下,靶向实验组和平行对照组U251细胞均呈黄绿色,非靶向对照组U251细胞则无显像. 结论 成功制备了功能化nano-GO-Tf-FITC微粒,并且其对脑胶质瘤U251细胞具有明显靶向显像作用.
目的 製備功能化納米氧化石墨烯(nano-GO-Tf-FITC)微粒,併研究其在近紅外線(NIR)照射下對腦膠質瘤U251細胞的靶嚮熒光顯像作用. 方法 將氧化石墨烯(GO)超聲振盪製得納米單層GO(nano-GO)微粒,以聚賴氨痠疊氮基糰(poly-L-lysine-G3)連接靶嚮分子轉鐵蛋白(Tf)和熒光分子異硫氰痠熒光素(FITC),製備齣功能化nano-GO-Tf-FITC微粒,應用透射電鏡在一定倍數下觀察併拍攝樣品形貌,測定樣品粒徑.將培養的腦膠質瘤U251細胞分成3組,即靶嚮實驗組(加入nano-GO-Tf-FITC微粒孵育)、平行對照組(加入抗CD71-FITC試劑孵育)和非靶嚮對照組(加入nano-GO-FITC微粒孵育),採用熒光顯微鏡檢測其熒光顯像. 結果 在高分辨率透射電鏡下nano-GO為20～100 nm單片層納米顆粒,功能化nano-GO-Tf-FIT微粒為分散在＜100 nm的單片層.在熒光顯微鏡下,靶嚮實驗組和平行對照組U251細胞均呈黃綠色,非靶嚮對照組U251細胞則無顯像. 結論 成功製備瞭功能化nano-GO-Tf-FITC微粒,併且其對腦膠質瘤U251細胞具有明顯靶嚮顯像作用.
목적 제비공능화납미양화석묵희(nano-GO-Tf-FITC)미립,병연구기재근홍외선(NIR)조사하대뇌효질류U251세포적파향형광현상작용. 방법 장양화석묵희(GO)초성진탕제득납미단층GO(nano-GO)미립,이취뢰안산첩담기단(poly-L-lysine-G3)련접파향분자전철단백(Tf)화형광분자이류청산형광소(FITC),제비출공능화nano-GO-Tf-FITC미립,응용투사전경재일정배수하관찰병박섭양품형모,측정양품립경.장배양적뇌효질류U251세포분성3조,즉파향실험조(가입nano-GO-Tf-FITC미립부육)、평행대조조(가입항CD71-FITC시제부육)화비파향대조조(가입nano-GO-FITC미립부육),채용형광현미경검측기형광현상. 결과 재고분변솔투사전경하nano-GO위20～100 nm단편층납미과립,공능화nano-GO-Tf-FIT미립위분산재＜100 nm적단편층.재형광현미경하,파향실험조화평행대조조U251세포균정황록색,비파향대조조U251세포칙무현상. 결론 성공제비료공능화nano-GO-Tf-FITC미립,병차기대뇌효질류U251세포구유명현파향현상작용.
Objective To prepare the functionalized nano-graphene oxide (nano-GO) particles,and study their targeted fluorescence imaging effects on glioma U251 cells under near infrared (NIR)irradiation.Methods Single-layer nano-GO particles were obtained after ultrasonic oscillation,and then connected with transferrin (Tf) and fluorescein isothiocyanate (FITC) through poly-L-lysine-G3 to prepare functionalized nano-GO-Tf-FITC particles.Sample morphology was observed and the particle sizes were measured under certain transmission electron microscope (TEM).Glioma U251 cells were employed and divided into targeted experimental group (adding functionalized nano-GO-Tf-FITC particles),parallel control group (adding anti-CD71-FITC reagent) and non-targeted experimental group (adding nano-GO-FITC particles).Fluorescence imaging of these groups was determined by fluorescence microscopy.Results Single-layer particles with a range of 20-100 nm were observed under high TEM,and the functionalized nano-GO-Tf-FITC particles were distributed in a pattern of single layer with a diameter smaller than 100 nm.Yellow green color was monitored in the targeted experimental group and parallel control group,and no imaging was monitored in the non-targeted experimental group because of no transferring integration.Conclusion Functionalized nano-GO-Tf-FITC particles are successfully prepared,and show marked targeted imaging effects on glioma U251 cells.
