CAJ | 학술논문

目的探索原矾酸钠(SOV)能否增强骨骼肌的运动功能及其可能的作用机制. 方法以73只mdx鼠为研究对象,按照SOV注射剂量的不同将其分为0 mg/kg(阴性对照组)及3、9、12、15、18和21 mg/kg SOV治疗组,另选10只C57BL/10小鼠作为正常对照组.连续尾静脉注射SOV 2次后,用生物机能实验系统BL-420E检测mdx鼠骨骼肌运动功能的改变,并分别用CK试剂盒、HE染色和免疫组化检测各组血清肌酸激酶(CK)值、肌肉病理特点和dystrophin表达. 结果与阴性对照组比较,SOV12和SOV15两组胫前肌的肌肉收缩力升高了20％～30％,SOV9、SOV12和SOV15组的运动单位动作电位(MUAP)波宽减小了约50％,治疗组MUAP波幅最高增大1.8mV,SOV12和SOV15组的MUAP相数减少至2相,差异有统计学意义(P＜0.05).与阴性对照组比较,SOV9、SOV12和SOV15组血清CK值明显降低(最高可达65％),差异有统计学意义(P＜0.05).与阴性对照组比较,SOV9、SOV12和SOV15组的肌肉病理改善最为明显,核中移肌纤维的数量降至52％-58％,差异有统计学意义(P＜0.05);而这3组炎性细胞的浸润也有一定程度的减轻. 结论 SOV能通过抑制Na+-K+-ATP酶的活性改善肌肉的运动功能,降低血清CK值,缓解肌纤维的变性坏死和炎性细胞浸润,提示其可能成为潜在治疗肌肉疾病的药物.
목적 탐색원반산납(SOV)능부증강골격기적운동공능급기가능적작용궤제. 방법 이73지mdx서위연구대상,안조SOV주사제량적불동장기분위0 mg/kg(음성대조조)급3、9、12、15、18화21 mg/kg SOV치료조,령선10지C57BL/10소서작위정상대조조.련속미정맥주사SOV 2차후,용생물궤능실험계통BL-420E검측mdx서골격기운동공능적개변,병분별용CK시제합、HE염색화면역조화검측각조혈청기산격매(CK)치、기육병리특점화dystrophin표체. 결과 여음성대조조비교,SOV12화SOV15량조경전기적기육수축력승고료20％～30％,SOV9、SOV12화SOV15조적운동단위동작전위(MUAP)파관감소료약50％,치료조MUAP파폭최고증대1.8mV,SOV12화SOV15조적MUAP상수감소지2상,차이유통계학의의(P＜0.05).여음성대조조비교,SOV9、SOV12화SOV15조혈청CK치명현강저(최고가체65％),차이유통계학의의(P＜0.05).여음성대조조비교,SOV9、SOV12화SOV15조적기육병리개선최위명현,핵중이기섬유적수량강지52％-58％,차이유통계학의의(P＜0.05);이저3조염성세포적침윤야유일정정도적감경. 결론 SOV능통과억제Na+-K+-ATP매적활성개선기육적운동공능,강저혈청CK치,완해기섬유적변성배사화염성세포침윤,제시기가능성위잠재치료기육질병적약물.
Objective To explore whether sodium orthovanadate (SOV) can improve the motor function of skeletal muscle and its mechanism.Methods Seventy-three mdx mice were chosen in our study and divided into negative control group,and 3,9,12,15,18 and 21 mg/kg SOV treatment groups according to the injection dosages of SOV; another 10 C57BL/10 mice were chosen as healthy control group.After twice injection,the changes of their motor functions were detected by Biological and Functional Experimental System BL-420E.In addition,creatine kinase (CK) reagent kit,HE staining and immunohistochemistry were used to observe the serum CK level,pathology features of skeletal muscles and dystrophin expression.Results As compared with that in the mdx mice,the contractility oftibialis anterior (TA) muscles in the 12 and 15 mg/kg SOV treatment groups obviously improved 20％-30％,the duration of motor unit action potential (MUAP) in 9,12 and 15 mg/kg SOV treatment groups was significantly shortened about 50％,the amplitude in all the treatment groups was enlarged to 1.8 mV and the phases in the 12 and 15 mg/kg SOV treatment groups decreased to 2 phases,with significant differences (P＜0.05).The serum CK level in the 9,12 and 15 mg/kg SOV treatment groups was significantly decreased,some reaching to 65％,as compared with that in the mdx mice.The central nucleation in the 9,12 and 15 mg/kg SOV treatment groups was significantly alleviated as compared with that in the mdx mice (P＜0.05); and the inflammation infiltration of involved muscles in mdx mice was severer.However,the unconformity of muscular fibers did not ameliorate and the dystrophin positive fibers did not increase in the SOV treatment groups as compared with those in mdx mice (P＞0.05).Conclusion SOV cannot improve the the motor function of mdx mice through inhibiting Na+-K+-ATPase activity,decrease the CK level and relieve the muscle fiber necrosis and inflammatory cell infiltration,therefore,SOV can probably be a potential medicine for muscular diseases in the future.