中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2011年
6期
432-435
,共4页
刘思隽%谢有富%戴丽冰%杜高伟
劉思雋%謝有富%戴麗冰%杜高偉
류사준%사유부%대려빙%두고위
瘢痕%促肾上腺皮质素释放激素%糖皮质激素
瘢痕%促腎上腺皮質素釋放激素%糖皮質激素
반흔%촉신상선피질소석방격소%당피질격소
Cicatrix%Corticotropin releasing hormone%Glucoeorticoids%Hypothalamus-pituitary-adrenal axis
目的 探讨增生性瘢痕组织中下丘脑-垂体-肾上腺皮质(HPA)轴成分的表达及意义.方法 应用实时荧光定量PCR法,检测人增生性瘢痕(12例)和正常皮肤(7例)组织中HPA轴的活性物质促肾上腺皮质素释放激素(CRH)、促肾上腺皮质素释放激素受体1(CRH-R1)、阿片-促黑素细胞皮质素原(POMC)、黑素皮质素受体2(MC-2R)以及糖皮质激素受体α(GR-α)的mRNA表达差异,免疫组织化学法观察CRH、CRH-R1、促肾上腺皮质激素(ACTH)、MC-2R以及GR-α的分布差异.对数据进行独立样本t检验. 结果 PCR结果显示,增生性瘢痕组织标本中CRH、CRH-R1、POMC及GR-α的mRNA表达分别为3.1±0.8、0.05±0.03、0.020±0.007、0.0000±0.0010,均低于正常皮肤组织的20.6±4.7、0.30±0.12、0.060±0.020、0.0200±0.0070,差异有统计学意义(t值为2.10-4.75,P值均小于0.05)；但2种组织中MC-2R mRNA表达水平接近(t=1.48,P=0.15).免疫组织化学法检测结果显示,CRH,CRH-R1、ACTH、MC-2R以及GR-α分布于瘢痕组织的表皮基底层、真皮层的Fb和汗腺管壁中.在正常皮肤组织中阳性细胞除分布于上述部位外,还可见于毛囊和皮脂腺. 结论 增生性瘢痕形成与组织中HPA轴的活性物质表达降低有关.
目的 探討增生性瘢痕組織中下丘腦-垂體-腎上腺皮質(HPA)軸成分的錶達及意義.方法 應用實時熒光定量PCR法,檢測人增生性瘢痕(12例)和正常皮膚(7例)組織中HPA軸的活性物質促腎上腺皮質素釋放激素(CRH)、促腎上腺皮質素釋放激素受體1(CRH-R1)、阿片-促黑素細胞皮質素原(POMC)、黑素皮質素受體2(MC-2R)以及糖皮質激素受體α(GR-α)的mRNA錶達差異,免疫組織化學法觀察CRH、CRH-R1、促腎上腺皮質激素(ACTH)、MC-2R以及GR-α的分佈差異.對數據進行獨立樣本t檢驗. 結果 PCR結果顯示,增生性瘢痕組織標本中CRH、CRH-R1、POMC及GR-α的mRNA錶達分彆為3.1±0.8、0.05±0.03、0.020±0.007、0.0000±0.0010,均低于正常皮膚組織的20.6±4.7、0.30±0.12、0.060±0.020、0.0200±0.0070,差異有統計學意義(t值為2.10-4.75,P值均小于0.05)；但2種組織中MC-2R mRNA錶達水平接近(t=1.48,P=0.15).免疫組織化學法檢測結果顯示,CRH,CRH-R1、ACTH、MC-2R以及GR-α分佈于瘢痕組織的錶皮基底層、真皮層的Fb和汗腺管壁中.在正常皮膚組織中暘性細胞除分佈于上述部位外,還可見于毛囊和皮脂腺. 結論 增生性瘢痕形成與組織中HPA軸的活性物質錶達降低有關.
목적 탐토증생성반흔조직중하구뇌-수체-신상선피질(HPA)축성분적표체급의의.방법 응용실시형광정량PCR법,검측인증생성반흔(12례)화정상피부(7례)조직중HPA축적활성물질촉신상선피질소석방격소(CRH)、촉신상선피질소석방격소수체1(CRH-R1)、아편-촉흑소세포피질소원(POMC)、흑소피질소수체2(MC-2R)이급당피질격소수체α(GR-α)적mRNA표체차이,면역조직화학법관찰CRH、CRH-R1、촉신상선피질격소(ACTH)、MC-2R이급GR-α적분포차이.대수거진행독립양본t검험. 결과 PCR결과현시,증생성반흔조직표본중CRH、CRH-R1、POMC급GR-α적mRNA표체분별위3.1±0.8、0.05±0.03、0.020±0.007、0.0000±0.0010,균저우정상피부조직적20.6±4.7、0.30±0.12、0.060±0.020、0.0200±0.0070,차이유통계학의의(t치위2.10-4.75,P치균소우0.05)；단2충조직중MC-2R mRNA표체수평접근(t=1.48,P=0.15).면역조직화학법검측결과현시,CRH,CRH-R1、ACTH、MC-2R이급GR-α분포우반흔조직적표피기저층、진피층적Fb화한선관벽중.재정상피부조직중양성세포제분포우상술부위외,환가견우모낭화피지선. 결론 증생성반흔형성여조직중HPA축적활성물질표체강저유관.
Objective To explore the expression and significance of secretions of hypothalamus-pituitary-adrenal (HPA) axis in human hypeorophic scar.Methods Hypertrphic scar tissues obtained from 12 patients with deep-partial thickness burn or full-thickness burn and normal skin tissues from the same 7 patients with hypertrophic scar were harvested for determination of gene expression of corticotrophinreleasing hormone (CRH),CRH receptor 1 (CRH-R1),pro-opiomelanocortin (POMC),melanocortin receptor 2 ( MC-2R),and glucocorticoid receptor α ( GR-α ) by real-time fluorecence quantitative PCR.After addition of corresponding antibodies,distribution differences of CRH,CRH-R1,adrenocorticotropic hormone ( ATCH ),MC-2R,and CR-α were observed with immunohistochemical staining,Data were processed with t test.Results The mRNA expression of CRH,CRH-R1,POMC,and GR-α in hypertrophie scar was respectively 3.1 ± 0.8,0.05 ± 0.03,0.020 ± 0.007,and 0.0030 ± 0.0010,which were significantly lower than those in normal skin ( 20.6 ± 4.7,0.30 ± 0.12,0.060 ± 0.020,and 0.0200 ± 0.0070,with t values from 2.10 to 4.75,P values all below 0.05 ).There was no statistical difference in MC-2R mRNA expression between hypertrophic scar and normal skin ( t=1.48,P =0.15).Immunohiatochemical observation showed CRH,CRH-R1,ACTH,MC-2R,and GR-α in hypertphic scar were located in basal layer of epidermis,fibroblast of dermis,and tube wall of sweat gland.Expressions of these indexes could also be observed in sebaceous gland and hair follicle besides above-mentioned structures.Conclusions Decreasing expression of active material of HPA axis may be related to formation of hypertrophic scar.
