CAJ | 학술논문

目的了解三黄生肤油对兔Ⅲ度烫伤创面的作用. 方法于9只实验兔背部左右两侧用水蒸气各制作l处Ⅲ度烫伤创面(面积为6 cm2).采用随机数字表法将兔分为麻油组、三黄生肤油组、莫匹罗星组,每组3只共6处创面,相应行麻油、三黄生肤油、莫匹罗星软膏外敷治疗,0.15 mL/cm2,2～3次/d.伤后1、11、22、45 d观察各组创面大体变化,记录创面愈合时间；伤后5、11、15、22 d计算创面愈合率.伤后第45天处死各组兔,取部分创面组织HE染色后行组织形态学观察；部分创面组织进行免疫荧光染色,检测bFGF、TGF-β1和血管内皮生长因子(VEGF)的表达.对数据进行单因素方差分析或LSD-t检验. 结果 (1)伤后l～45 d,三黄生肤油组创面愈合情况优于其他2组.(2)与麻油组[(21.2±3.1)d]相比,三黄生肤油组创面愈合时间明显缩短[(11.2±2.3)d,t =2.591,P＜0.05].(3)伤后5～22 d各组创面愈合率持续升高；三黄生肤油组各时相点创面愈合率明显高于麻油组(t值为3.920～8.605,P值均小于0.05).(4)HE染色结果显示,与麻油组和莫匹罗星组相比,三黄生肤油组创面结构完整,真皮层可见新生致密的毛囊,新生胶原纤维排列整齐；同时免疫荧光染色显示三黄生肤油组bFGF、TGF-β1及VEGF蛋白表达水平较高. 结论三黄生肤油可促进创面组织bFGF、TGF-β1及VEGF蛋白表达的上调,诱导新生血管形成,加快创面修复,缩短创面愈合时间.
목적 료해삼황생부유대토Ⅲ도탕상창면적작용. 방법 우9지실험토배부좌우량측용수증기각제작l처Ⅲ도탕상창면(면적위6 cm2).채용수궤수자표법장토분위마유조、삼황생부유조、막필라성조,매조3지공6처창면,상응행마유、삼황생부유、막필라성연고외부치료,0.15 mL/cm2,2～3차/d.상후1、11、22、45 d관찰각조창면대체변화,기록창면유합시간；상후5、11、15、22 d계산창면유합솔.상후제45천처사각조토,취부분창면조직HE염색후행조직형태학관찰；부분창면조직진행면역형광염색,검측bFGF、TGF-β1화혈관내피생장인자(VEGF)적표체.대수거진행단인소방차분석혹LSD-t검험. 결과 (1)상후l～45 d,삼황생부유조창면유합정황우우기타2조.(2)여마유조[(21.2±3.1)d]상비,삼황생부유조창면유합시간명현축단[(11.2±2.3)d,t =2.591,P＜0.05].(3)상후5～22 d각조창면유합솔지속승고；삼황생부유조각시상점창면유합솔명현고우마유조(t치위3.920～8.605,P치균소우0.05).(4)HE염색결과현시,여마유조화막필라성조상비,삼황생부유조창면결구완정,진피층가견신생치밀적모낭,신생효원섬유배렬정제；동시면역형광염색현시삼황생부유조bFGF、TGF-β1급VEGF단백표체수평교고. 결론 삼황생부유가촉진창면조직bFGF、TGF-β1급VEGF단백표체적상조,유도신생혈관형성,가쾌창면수복,축단창면유합시간.
Objective To observe the effect of San-huang-sheng-fu oil on wounds of full-thickness scald in rabbits.Methods Full-thickness scald wounds with area of 6 cm2 were reproduced on both sides of the back in 9 experimental rabbits by water vapor.These rabbits were divided into sesame oil (S1),Sanhuang-sheng-fu oil (S2),and mupirocin ointment (M) groups according to the random number table,with 3 rabbits (6 wounds) in each group.Two wounds of each rabbit in the three groups were respectively treated with sesame oil,San-huang-sheng-fu oil,and mupirocin ointment,in a dose of 0.15 mL/cm2,2-3 times per day.The general condition of wounds was observed on post scald day (PSD) 1,11,22,and 45.The wound healing time was recorded.The wound healing rate was calculated on PSD 5,11,15,and 22.All the rabbits were sacrificed on PSD 45,and wound tissues were subjected to histomorphological study with HE staining.The protein expressions of transforming growth factor β1 (TGF-β1),basic fibroblast growth factor (bFGF),and vascular endothelial growth factor (VEGF) were observed with immunofluorescence staining for the other part of wound tissues.Data were processed with one-way analysis of variance or LSD-t test.Results (1) The wound healing quality of rabbits in S2 group was better than that in the other two groups.(2) The wound healing time of rabbits in S2 group [(11.2 ± 2.3) d] was significantly shorter than that in S1 group [(21.2±3.1) d,t =2.591,P ＜0.05].(3) The wound healing rate of rabbit in each group was increased gradually on PSD 5-22.The wound healing rates of rabbits in S2 group on PSD 5-22 were significantly higher than those in S1 group (with t values from 3.920 to 8.605,P values all below 0.05).(4)Histomorphological observation showed that the structure of wound tissues in S2 group was in much better integrity than that in the other two groups,including regenerated hair follicles in the corium layer and regularly arranged collagen fibers.The protein expressions of TGF-β1,bFGF,and VEGF in S2 group were all higher than those in the other two groups.Conclusions San-huang-sheng-fu oil can up-regulate the protein expressions of TGF-β1,bFGF,and VEGF,induce vascular regeneration,promote wound healing,and shorten wound healing time.