CAJ | 학술논문

目的建立产瘫和成人臂丛神经根性损伤出现垂腕垂指畸形的大鼠模型.方法取雄性SD大鼠48只,其中幼年组(生后7 d)和成年组(大于3月龄)各24只,每个年龄组大鼠按神经根处理方式等分成3个亚组,即切断右侧C5、6神经根组、切断C5、6、7神经根组和切断C5、6、7、8神经根组.术后3个月后行如下检测:各组大鼠垂腕垂指发生率；桡神经有髓神经纤维通过率；Western Blot检测桡神经组织内神经丝蛋白200(NF200)和外周髓鞘蛋白0(MPZ)的表达水平.结果 C5、6神经根切断后,各年龄组大鼠实验侧均无垂腕垂指畸形,桡神经有髓神经纤维的通过率较健侧均无明显降低,各组间差异无统计学意义(P＞0.05).各组桡神经组织内NF200和MPZ相对表达量差异均无统计学意义(P＞0.05).C5、6、7神经根切断后,幼年组垂腕垂指发生率均明显高于成年组(P＜0.05),桡神经有髓神经纤维通过率均低于成年组(P＜0.05).幼年组桡神经组织内NF200和MPZ相对表达量均低于成年组(P＜0.05).C5、6、7、8切断后,各年龄组大鼠每例实验侧均出现垂腕垂指畸形,各组实验侧桡神经有髓神经纤维通过率均明显降低,但无年龄差异(P＞0.05).各年龄组桡神经组织内NF200和MPZ相对表达量均降低,但差异无统计学意义(P＞0.05).结论幼年及成年大鼠臂丛神经根性损伤造成垂腕垂指畸形的规律与人类相似,幼年大鼠C5、6、7神经根断裂即可导致垂腕垂指,而成年大鼠只有连续4个神经根(C5、6、7、8)断裂才会导致垂腕垂指畸形.因此大鼠是进行产瘫与成人臂丛神经根性损伤垂腕垂指畸形发生机制研究的理想动物.
목적 건립산탄화성인비총신경근성손상출현수완수지기형적대서모형.방법 취웅성SD대서48지,기중유년조(생후7 d)화성년조(대우3월령)각24지,매개년령조대서안신경근처리방식등분성3개아조,즉절단우측C5、6신경근조、절단C5、6、7신경근조화절단C5、6、7、8신경근조.술후3개월후행여하검측:각조대서수완수지발생솔；뇨신경유수신경섬유통과솔；Western Blot검측뇨신경조직내신경사단백200(NF200)화외주수초단백0(MPZ)적표체수평.결과 C5、6신경근절단후,각년령조대서실험측균무수완수지기형,뇨신경유수신경섬유적통과솔교건측균무명현강저,각조간차이무통계학의의(P＞0.05).각조뇨신경조직내NF200화MPZ상대표체량차이균무통계학의의(P＞0.05).C5、6、7신경근절단후,유년조수완수지발생솔균명현고우성년조(P＜0.05),뇨신경유수신경섬유통과솔균저우성년조(P＜0.05).유년조뇨신경조직내NF200화MPZ상대표체량균저우성년조(P＜0.05).C5、6、7、8절단후,각년령조대서매례실험측균출현수완수지기형,각조실험측뇨신경유수신경섬유통과솔균명현강저,단무년령차이(P＞0.05).각년령조뇨신경조직내NF200화MPZ상대표체량균강저,단차이무통계학의의(P＞0.05).결론 유년급성년대서비총신경근성손상조성수완수지기형적규률여인류상사,유년대서C5、6、7신경근단렬즉가도치수완수지,이성년대서지유련속4개신경근(C5、6、7、8)단렬재회도치수완수지기형.인차대서시진행산탄여성인비총신경근성손상수완수지기형발생궤제연구적이상동물.
Objective To establish the animal model for simulating carpoptosis after birth palsy and adult brachial plexus root injury.Methods Forty-eight male Sprague-Dawley rats were divided,based on their ages,into two groups of 24 each,i.e.,pup group at the age of 7 days postnatal (P7) and adult group (over 3months old).Each group was equally subdivided into three for performing the rupture of the right C5,6,C5,6,7 and C5,6,7,8 nerve roots respectively.In the 3rd month postoperatively,the following tests were performed:the incidence of carpoptosis in each group,the through put of the myelinated fibers in the radial nerve.The expression level of NF200 and myelin protein P0(MPZ) of the affected radial nerve were measured by Western Blot.Results After C5,6 were ruptured,there was no carpoptosis in each age group.There was no difference of the through put of radial myelinated fibers between in pup group and adult group( P ＞ 0.05).There was no difference of the expression level of NF200 and MPZ between pup group and adult group( P ＞ 0.05).After C5,6,7were ruptured,the incidence of carpoptosis in pup group was higher than that of the adult group( P ＜ 0.05),the through put of radial myelinated fibrers in pup group was lower than that of the adult group( P ＜ 0.05) and the expression level of NF200 and MPZ in pup group was lower tlan that of the adult group( P ＜ 0.05).After the C5,6,7,8 were ruptured,all these rats represented carpoptosis.The through put of radial myelinated fiber in both groups were decreased but there was no statistically significant difference( P ＞ 0.05).There was no difference between the expression level of NF200 and MPZ in pup group and adult group( P ＞ 0.05).Conclusion The occurrence pattern of carpoptosis caused by root injury of brachial plexus in pup and adult rats is similar to that of the newborn and adult.That is to say,C5,6,7 root injury can lead to carpoptosis in pup rats while only four consecutive nerve(C5,6,7,8) roots involved can cause carpoptosis in adult rats.Therefore the rat is an ideal animal for studies of the mechanism of wrist drop caused by birth palsy and adult root injury of brachial plexus.