CAJ | 학술논문

目的评价应用硫酸软骨素酶ABC降解硫酸软骨素蛋白多糖的去细胞异体神经修复神经缺损的效果.方法经硫酸软骨素酶ABC处理后化学去细胞异体神经修复大鼠15 mm坐骨神经缺损为实验组(A组),对照组为未经酶处理的化学去细胞异体神经移植组(B组)和自体神经移植组(C组).术后8、12周进行痛觉刺激试验.术后12周进行肌电图检查,检测小腿三头肌湿重和有髓神经纤维密度,评价其修复周围神经缺损的效果.结果术后8周,A组痛觉恢复率高于B、C组.术后12周A、C组痛觉均恢复,B组部分大鼠痛觉恢复.术后12周,A、C组小腿三头肌湿重差异无统计学意义,移植段神经5mm处有髓神经纤维密度A组高于C组,移植段神经10 mm处有髓神经纤维密度差异无统计学意义,移植段神经以远5 mm处胫神经平面有髓神经纤维密度A组略低于C组.肌电图动作电位波幅A组和C组无明显差别.神经传导速度A组低于C组.A、C组上述指标均优于B组.结论经硫酸软骨素酶ABC处理的去细胞神经,有利于轴突进人生长,缩短靶器官神经重新支配时间,提高修复效果.
목적 평개응용류산연골소매ABC강해류산연골소단백다당적거세포이체신경수복신경결손적효과.방법 경류산연골소매ABC처리후화학거세포이체신경수복대서15 mm좌골신경결손위실험조(A조),대조조위미경매처리적화학거세포이체신경이식조(B조)화자체신경이식조(C조).술후8、12주진행통각자격시험.술후12주진행기전도검사,검측소퇴삼두기습중화유수신경섬유밀도,평개기수복주위신경결손적효과.결과 술후8주,A조통각회복솔고우B、C조.술후12주A、C조통각균회복,B조부분대서통각회복.술후12주,A、C조소퇴삼두기습중차이무통계학의의,이식단신경5mm처유수신경섬유밀도A조고우C조,이식단신경10 mm처유수신경섬유밀도차이무통계학의의,이식단신경이원5 mm처경신경평면유수신경섬유밀도A조략저우C조.기전도동작전위파폭A조화C조무명현차별.신경전도속도A조저우C조.A、C조상술지표균우우B조.결론 경류산연골소매ABC처리적거세포신경,유리우축돌진인생장,축단파기관신경중신지배시간,제고수복효과.
Objective To evaluate the effect of chondrotinase ABC treated decelluarilzed nerve allograft on repairing never defects in rats.Methods Nerve allografts were treated with chondrotinase ABC and decellurized using chemical detergents.The allografts were employed to repair 15 mm sciatic nerve defects in rats in the experiment group (Group A).The nerve gaps were bridged by decellularized nerve without chondrotinase ABC treatment in Group B and by autografts in Group C.Pain reaction was tested at 8 weeks and 12 weeks postoperatively.Electrophysiology,muscle wet weight of the gastrocnemius-soleus complex and the myelinated fiber density of the regenerated nerve were quantified at 12 weeks postoperatively.Results The proportion of pain reaction recovery in Group A was higher than that in Group B and Group C at 8 weeks postoperatively.At 12 weeks postoperatively,pain reaction test was positive in all the rats in Group A and Group C,whereas most of but not all the rats in Group B had a positive pain reaction.There was no significant difference in muscle weight of rats in Group A and Group C at 12 weeks.The myelinated nerve fiber density at the proximal 5 mm of the graft was higher in Group A than in Group C,whereas no significant difference was seen at the distal 10 mm of the graft.The myelinated nerve fiber density was slight lower in Group A than in Group C in the tibial nerve division 5 mm distal to the graft.Compound muscle action potential amplitude showed no differences between group A and group C.Nerve conduction velocity was slower in Group A than in Group C.The aforementioned indexes in Group B were inferior to Group A and Group C.Conclusion Chondrotinase ABC treatment facilitates axons to enter the decelluarilzed nerve allograft.This reduces target organ reinnervation time and improves functional recovery.