CAJ | 학술논문

目的研究当归多糖(angelica polysaccharides,ApS)对大鼠皮瓣缺血再灌注损伤后血液流变学指标的影响以及对损伤的保护作用.方法取50只SD大鼠随机分为五组:假处理组、对照组、2 mg/kg APS组、4mg/kg APS组和8mg/kg APS组.制取5 cm×4 cm腹部岛状皮瓣后,假处理组不作缺血-再灌注处理而直接原位缝合,对照组和各APS组缺血6小时后再灌注.各APS组于再灌注前5 min腹腔注射相应剂量APS 0.2 ml,对照组则注射生理盐水0.2ml.于再灌注12 h后测定三组大鼠皮瓣组织丙二醛(malondialdehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)水平、计数外周血中性粒细胞并用流式细胞术测定其表面CD1 1a/CD18和CD11 b/CD18表达水平、检测外周血血液流变学各指标.结果再灌注12 h后,8mg/kg APS组与对照组相比,皮瓣MDA含量和CD11a/CD18、CD1 1b./CD18表达明显下降(P＜0.05),SOD水平显著升高(P＜0.05)；各APS组外周血中性粒细胞数量,全血低切粘度、中切粘度和高切粘度均明显低于对照组(P＜0.05)；各APS组较对照组血浆粘度、红细胞聚集指数差异无统计学意义(P＞0.05).结论 APS通过减少中性粒细胞数量,下调中性粒细胞表面的CD11 a/CD18和CD11b/CD18表达,降低全血粘度来减轻大鼠皮瓣缺血再灌注损伤.
목적 연구당귀다당(angelica polysaccharides,ApS)대대서피판결혈재관주손상후혈액류변학지표적영향이급대손상적보호작용.방법 취50지SD대서수궤분위오조:가처리조、대조조、2 mg/kg APS조、4mg/kg APS조화8mg/kg APS조.제취5 cm×4 cm복부도상피판후,가처리조불작결혈-재관주처리이직접원위봉합,대조조화각APS조결혈6소시후재관주.각APS조우재관주전5 min복강주사상응제량APS 0.2 ml,대조조칙주사생리염수0.2ml.우재관주12 h후측정삼조대서피판조직병이철(malondialdehyde,MDA)화초양화물기화매(superoxide dismutase,SOD)수평、계수외주혈중성립세포병용류식세포술측정기표면CD1 1a/CD18화CD11 b/CD18표체수평、검측외주혈혈액류변학각지표.결과 재관주12 h후,8mg/kg APS조여대조조상비,피판MDA함량화CD11a/CD18、CD1 1b./CD18표체명현하강(P＜0.05),SOD수평현저승고(P＜0.05)；각APS조외주혈중성립세포수량,전혈저절점도、중절점도화고절점도균명현저우대조조(P＜0.05)；각APS조교대조조혈장점도、홍세포취집지수차이무통계학의의(P＞0.05).결론 APS통과감소중성립세포수량,하조중성립세포표면적CD11 a/CD18화CD11b/CD18표체,강저전혈점도래감경대서피판결혈재관주손상.
Objective To investigate the hemorheology changes in flap ischemia reperfusion injury and the protective effect of angelica polysaccharides (APS).Methods Fifty adult SD rats were divided into a sham group,a control group,a 2 mg/kg APS group,a 4 mg/kg APS group,and a 8 mg/kg APS group.After a 5 cm × 4 cm epigastric flap was elevated,flaps in the sham group were sutured back into its place without inducing any ischemia.Flaps in the control group and APS treatment groups were subject to a 6-hour ischemic period.Five minutes prior to the repeffusion,0.2 ml saline was injected via intraperitoneal route in the control group while 0.2 ml of corresponding doses of angelica polysaccharides were injected in the APS groups.Following a 12-hour reperfusion,the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the flap tissue were measured.Polymorphonuclear neutrophils (PMN) in the peripheral blood were counted.Flow cytometry was used to detect the expression of CD11a/CD18 and CD11b/CD18 on the polynorphonuclear neutrophils.Hemorheology parameters were detected in the rats of each group.Results Compared with the control group,8 mg/kg APS group showed significant decrease of MDA content and CD11a/CD18,CD11b/CD18 expression levels after 12-hour repeffusion (P ＜ 0.05).The level of SOD increased significantly (P ＜ 0.05).The PMN number,the low shear viscosity,the middle shear viscosity and high shear viscosity of the APS groups were significantly lower than those of the control group (P ＜ 0.05).Plasma viscosity,RBC aggregation index of the APS groups were not significantly different from the control group (P ＞ 0.05).Conclusion APS can reduce ischemia reperfusion injury of skin flaps in rats via reducing the number of neutrophils,down-regulating the expression of CD11a/CD18 and CD11b/CD18 on neutrophil surface,and decreasing blood viscosity,so as to improve blood supply of the flap.