中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2009年
5期
378-380
,共3页
卢文波%姜智南%陈顺美%覃世榕%赵玲军%杨海涛%施丹华%陈雪梅
盧文波%薑智南%陳順美%覃世榕%趙玲軍%楊海濤%施丹華%陳雪梅
로문파%강지남%진순미%담세용%조령군%양해도%시단화%진설매
宫颈肿瘤%DNA%RNA%乳头状病病毒%人
宮頸腫瘤%DNA%RNA%乳頭狀病病毒%人
궁경종류%DNA%RNA%유두상병병독%인
Cervix neoplasms%DNA%RNA%Papillomavims,human
目的 研究在单一时间点上的人乳头瘤病毒(HPV)E6/E7 mRNA检测及HC2-HPV-DNA检测在宫颈疾病筛查中的应用.方法 对2008年1月至2009年7月就诊的130例患者进行HPV E6/E7 mRNA与HC2-HPV-DNA检测.以TCT结果为标准评价两种方法检测宫颈高度病变的效能.结果 HC2-HPV-DNA检测阳性检出率82.3%(107/130),HPV E6/E7 mRNA检测阳性检出率40.0%(52/130),两种方法在阳性检出方面差异有统计学意义(X2=24.5,P＜0.05).HC2-HPV-DNA检测宫颈高度病变的敏感性为90.1%、特异性为22.1%、阳性预测值为37.4%、阴性预测值为82.6%;HPV E6/E7mRNA检测宫颈高度病变的敏感性为65.9%、特异性为73.3%、阳性预测值为55.8%、阴性预测值为80.8%.结论 在单一时间点的宫颈癌筛查中结合HC2-HPV-DNA检测和HPV E6/E7 mRNA检测更具有潜在价值.
目的 研究在單一時間點上的人乳頭瘤病毒(HPV)E6/E7 mRNA檢測及HC2-HPV-DNA檢測在宮頸疾病篩查中的應用.方法 對2008年1月至2009年7月就診的130例患者進行HPV E6/E7 mRNA與HC2-HPV-DNA檢測.以TCT結果為標準評價兩種方法檢測宮頸高度病變的效能.結果 HC2-HPV-DNA檢測暘性檢齣率82.3%(107/130),HPV E6/E7 mRNA檢測暘性檢齣率40.0%(52/130),兩種方法在暘性檢齣方麵差異有統計學意義(X2=24.5,P＜0.05).HC2-HPV-DNA檢測宮頸高度病變的敏感性為90.1%、特異性為22.1%、暘性預測值為37.4%、陰性預測值為82.6%;HPV E6/E7mRNA檢測宮頸高度病變的敏感性為65.9%、特異性為73.3%、暘性預測值為55.8%、陰性預測值為80.8%.結論 在單一時間點的宮頸癌篩查中結閤HC2-HPV-DNA檢測和HPV E6/E7 mRNA檢測更具有潛在價值.
목적 연구재단일시간점상적인유두류병독(HPV)E6/E7 mRNA검측급HC2-HPV-DNA검측재궁경질병사사중적응용.방법 대2008년1월지2009년7월취진적130례환자진행HPV E6/E7 mRNA여HC2-HPV-DNA검측.이TCT결과위표준평개량충방법검측궁경고도병변적효능.결과 HC2-HPV-DNA검측양성검출솔82.3%(107/130),HPV E6/E7 mRNA검측양성검출솔40.0%(52/130),량충방법재양성검출방면차이유통계학의의(X2=24.5,P＜0.05).HC2-HPV-DNA검측궁경고도병변적민감성위90.1%、특이성위22.1%、양성예측치위37.4%、음성예측치위82.6%;HPV E6/E7mRNA검측궁경고도병변적민감성위65.9%、특이성위73.3%、양성예측치위55.8%、음성예측치위80.8%.결론 재단일시간점적궁경암사사중결합HC2-HPV-DNA검측화HPV E6/E7 mRNA검측경구유잠재개치.
Objective To research the application of the single time detection of HPV E6/E7 mRNA and HC2-HPV-DNA in cervical screening project.Methods We detected both HPV E6/E7 mRNA and HC2-HPVDNA of each sample which collected from 130 cervical disease patients' cervix during Jan.2008 and July.2009.TCT results were taken as standard to evaluate the diagnostic accuracy of the above two test methods in detecting high-grade cervical disease.Results 82.3%(107/130)women were confirmed to infect HPV by HC2-HPV-DNA detection,and 40.0% (52/130)women were confirmed to infect HPV by HPV E6/E7 mRNA detection,there was no significant difference between the two results(X2=24.5,P＜0.05).The sensitivity,specificity,positive predictive value,negative predictive value of HC2-HPV-DNA detection were 90.1 %,22.1%,37.4% and 82.6 %,respectively.The sensitivity,specificity,positive predictive value,negative predictive value of HPV E6/E7 mRNA detection were 65.9%,73.3%,55.8% and 80.8%,respectively.ConclusionIn clinical cervical screening project of single time,the combination of HC2-HPV-DNA detection and HPV E6/E7 mRNA detection wick take on more potential value than applying each of them alone.
