中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2011年
1期
2-4
,共3页
肖霓光%谢志萍%周琼华%张蓉芳%钟礼立%高寒春%丁小芳%李嘉%宋靖荣%侯云德%张兵%段招军
肖霓光%謝誌萍%週瓊華%張蓉芳%鐘禮立%高寒春%丁小芳%李嘉%宋靖榮%侯雲德%張兵%段招軍
초예광%사지평%주경화%장용방%종례립%고한춘%정소방%리가%송정영%후운덕%장병%단초군
呼吸道感染%冠状病毒HKU1%儿童,住院
呼吸道感染%冠狀病毒HKU1%兒童,住院
호흡도감염%관상병독HKU1%인동,주원
Respiratory tract infections%Coronavirus HKU1%Child,hospitalized
目的 了解冠状病毒HKU1在长沙地区急性下呼吸道感染住院儿童中的感染状况及临床特征.方法 采集2007年9月至2008年8月长沙地区因急性下呼吸道感染住院儿童鼻咽抽吸物(nasopharyngeal aspirates,NPA)样本1165份,用RT-PCR方法 检测冠状病毒HKU1 pol基因,测序分析阳性扩增产物,统计分析临床资料.结果 1165份NPA样本中,检测出12份冠状病毒HKU1,阳性率为1.03%(12/1165),患儿年龄最小8天,最大3岁,临床诊断多见支气管肺炎(83.33%),所测核苷酸序列与冠状病毒HKU1参考株同源性为98.18%～100%.结论 冠状病毒HKU1阳性患儿年龄均在3岁及3岁以下;性别无差异;冬春季检出率高;单一基因型在长沙地区流行;HKU1也是我国儿童急性下呼吸道感染中的一种病原.
目的 瞭解冠狀病毒HKU1在長沙地區急性下呼吸道感染住院兒童中的感染狀況及臨床特徵.方法 採集2007年9月至2008年8月長沙地區因急性下呼吸道感染住院兒童鼻嚥抽吸物(nasopharyngeal aspirates,NPA)樣本1165份,用RT-PCR方法 檢測冠狀病毒HKU1 pol基因,測序分析暘性擴增產物,統計分析臨床資料.結果 1165份NPA樣本中,檢測齣12份冠狀病毒HKU1,暘性率為1.03%(12/1165),患兒年齡最小8天,最大3歲,臨床診斷多見支氣管肺炎(83.33%),所測覈苷痠序列與冠狀病毒HKU1參攷株同源性為98.18%～100%.結論 冠狀病毒HKU1暘性患兒年齡均在3歲及3歲以下;性彆無差異;鼕春季檢齣率高;單一基因型在長沙地區流行;HKU1也是我國兒童急性下呼吸道感染中的一種病原.
목적 료해관상병독HKU1재장사지구급성하호흡도감염주원인동중적감염상황급림상특정.방법 채집2007년9월지2008년8월장사지구인급성하호흡도감염주원인동비인추흡물(nasopharyngeal aspirates,NPA)양본1165빈,용RT-PCR방법 검측관상병독HKU1 pol기인,측서분석양성확증산물,통계분석림상자료.결과 1165빈NPA양본중,검측출12빈관상병독HKU1,양성솔위1.03%(12/1165),환인년령최소8천,최대3세,림상진단다견지기관폐염(83.33%),소측핵감산서렬여관상병독HKU1삼고주동원성위98.18%～100%.결론 관상병독HKU1양성환인년령균재3세급3세이하;성별무차이;동춘계검출솔고;단일기인형재장사지구류행;HKU1야시아국인동급성하호흡도감염중적일충병원.
Objective In order to understand the epidemiological and virologic characteristics of coronavirus HKU1 infection in hospitalized children with acute respiratory tract infection (ARTI) in Changsha. Methods 1165 nasopharyngeal aspirates (NPA) specimens were collected from hospitalized children with ARTI between September 2007 and August 2008 in Changsha. Specimens were screened for pol gene of coronavirus HKU1 by polymerase chain reaction. All positive amplification products were confirmed by sequencing and compared with those in GenBank. Results Coronavirus HKU1 were detected in 12patients (1.03%) out of the 1165 children. The patients were from 8 days to 3 years. The most common clinical diagnosis was bronchopneumonia( 83. 33% ). Similarity of coronavirus HKU1 with those published in the GenBank at nucleotide levels was 98. 18%-100%. Conclusion Coronavirus HKU1 may be important pathogens in children with acute lower respiratory tract infection. Coronavirus HKU1 infections are common in children under 3 years old. There is no significant difference in the infectious rate between the boys and the girls. The peak of its prevalence is in spring and winter. A single genetic lineage of Coronavirus HKU1was revealed in human subjects in Changsha.
