中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2011年
1期
8-10
,共3页
周琼华%张兵%谢志萍%高寒春%郭铭伟%张菲%闫坤龙%张蓉芳%张静%曹长青%段招军
週瓊華%張兵%謝誌萍%高寒春%郭銘偉%張菲%閆坤龍%張蓉芳%張靜%曹長青%段招軍
주경화%장병%사지평%고한춘%곽명위%장비%염곤룡%장용방%장정%조장청%단초군
Saffold病毒%呼吸道感染%聚合酶链反应
Saffold病毒%呼吸道感染%聚閤酶鏈反應
Saffold병독%호흡도감염%취합매련반응
Saffold virus%Respiratory tract infections%Polymerase chain reaction
目的 了解长沙地区Saffold病毒(以下简称为SAFV)在儿童呼吸道感染中的流行情况,探讨其与儿童呼吸道感染的相关性.方法 选取湖南省人民医院儿科医学中心2007年11月至2008年10月间643名因呼吸道感染住院儿童的鼻咽抽吸物.采用实时荧光定量聚合酶链式反应(Real time-PCR)方法 扩增SAFV的5UTR的基因片段,并且统计分析临床资料.结果 643份样本中共检测出SAFV阳性67份,阳性检出率为10.42%(67/643),5岁以上未检测出该病毒.31例患迁延性肺炎和慢性肺炎的患儿标本检出SAFV 8例(25.81%),差异有统计学意义.结论 本研究表明长沙地区下呼吸道感染住院儿童存在SAFV感染;SAFV可能与下呼吸道感染及病程迁延相关.
目的 瞭解長沙地區Saffold病毒(以下簡稱為SAFV)在兒童呼吸道感染中的流行情況,探討其與兒童呼吸道感染的相關性.方法 選取湖南省人民醫院兒科醫學中心2007年11月至2008年10月間643名因呼吸道感染住院兒童的鼻嚥抽吸物.採用實時熒光定量聚閤酶鏈式反應(Real time-PCR)方法 擴增SAFV的5UTR的基因片段,併且統計分析臨床資料.結果 643份樣本中共檢測齣SAFV暘性67份,暘性檢齣率為10.42%(67/643),5歲以上未檢測齣該病毒.31例患遷延性肺炎和慢性肺炎的患兒標本檢齣SAFV 8例(25.81%),差異有統計學意義.結論 本研究錶明長沙地區下呼吸道感染住院兒童存在SAFV感染;SAFV可能與下呼吸道感染及病程遷延相關.
목적 료해장사지구Saffold병독(이하간칭위SAFV)재인동호흡도감염중적류행정황,탐토기여인동호흡도감염적상관성.방법 선취호남성인민의원인과의학중심2007년11월지2008년10월간643명인호흡도감염주원인동적비인추흡물.채용실시형광정량취합매련식반응(Real time-PCR)방법 확증SAFV적5UTR적기인편단,병차통계분석림상자료.결과 643빈양본중공검측출SAFV양성67빈,양성검출솔위10.42%(67/643),5세이상미검측출해병독.31례환천연성폐염화만성폐염적환인표본검출SAFV 8례(25.81%),차이유통계학의의.결론 본연구표명장사지구하호흡도감염주원인동존재SAFV감염;SAFV가능여하호흡도감염급병정천연상관.
Objective To investigate prevalence of Saffold virus (SAFV) in Changsha area of hospitalized children with respiratory tract infection, and to discuss whether this virus is related to respiratory tract infection of children. Methods 643 nasopharyngeal aspirates samples were collected from hospitalized children with respiratory tract infection of the first affiliated hospital of Hunan nomal university during Nov. 2007 to Oct. 2008. Real-time fluorescent quanti-tative PCR(FQ-PCR) performed to screen the 5'UTR gene.And then analyze clinical data. Results SAFV were detected in 67 patients ( 10.42% ) out of the 643children, it was not detected over 5 years of age. The virus were detected in 8 patients ( 25.81% ) out of the 31 children with persistent pneumonia and chronic pneumonia, there was statistically significant.Conclusion There existed SAFV infection in hospitalized children with lower respiratory infection in Changsha area;SAFV maybe related to disease onset with lower respiratory tract infection of children.
