CAJ | 학술논문

目的调查兰州地区5岁以下婴幼儿病毒性腹泻的流行情况,了解四种主要腹泻病毒在儿童中的分布情况.方法采集2009年7月至2010年6月兰州大学第一医院儿科5岁以下腹泻患儿粪便标本290份及儿童保健中心健康婴幼儿正常粪便标本114份,采用酶联免疫吸附试验(ELISA)检测轮状病毒抗原,采用巢式聚合酶链反应对轮状病毒阳性标本进行分型;采用反转录-聚合酶链反应(RT-PCR)检测杯状病毒和星状病毒,聚合酶链反应(PCR)检测腺病毒.结果 290份腹泻标本中四种病毒的阳性率分别为:轮状病毒39.31%,杯状病毒11.38%,腺病毒10.69%,星状病毒4.83%;对114份轮状病毒阳性标本G、P分型,G3型及P[8]型为优势株;114份正常标本轮状病毒检出率为0,杯状病毒检出7例,星状病毒检出1例,腺病毒检出5例.结论病毒性病原在兰州地区婴幼儿腹泻中占有重要地位,长期系统的监测具有重要意义.
목적 조사란주지구5세이하영유인병독성복사적류행정황,료해사충주요복사병독재인동중적분포정황.방법 채집2009년7월지2010년6월란주대학제일의원인과5세이하복사환인분편표본290빈급인동보건중심건강영유인정상분편표본114빈,채용매련면역흡부시험(ELISA)검측륜상병독항원,채용소식취합매련반응대륜상병독양성표본진행분형;채용반전록-취합매련반응(RT-PCR)검측배상병독화성상병독,취합매련반응(PCR)검측선병독.결과 290빈복사표본중사충병독적양성솔분별위:륜상병독39.31%,배상병독11.38%,선병독10.69%,성상병독4.83%;대114빈륜상병독양성표본G、P분형,G3형급P[8]형위우세주;114빈정상표본륜상병독검출솔위0,배상병독검출7례,성상병독검출1례,선병독검출5례.결론 병독성병원재란주지구영유인복사중점유중요지위,장기계통적감측구유중요의의.
Objective To study the epidemiologic characteristics of viral diarrhea in children under 5 years old in Lanzhou, understand the four major virus in children of distribution. Methods In the first hospital of Lanzhou university from Jul 2009 to Jun 2010 ,we collected 290 stool specimens from children with diarrhea and 114 asymptomatic controls. Rotavirus was detected by ELISA, further strain characterization was carried out by nested PCR. The human calicivirus, astrovirus, adenovirus were detected by RT- multiplex PCR and PCR. Results At least one of the four viral agents was found in 60% of the specimens.Rotavirus, human calicivirus, adenovirus, and astrovirus were identified in 39. 31% , 11.38 % , 10.69% , and 4.83% in 290 specimens respectively. Rotavirus G3 was the most prevailing serotype, P [8] was the most common genotype. In the 114 control samples, 7 sample was positived for calicivirus, 5 samples were positived for human adenovirus and 1 sample was positived for astrovirus. Conclusion The results indicated clearly the impact of viral agents causing diarrhea and the importance of long-term systematic surveillance.