CAJ | 학술논문

目的研究西藏自治区1999-2000年≤5岁儿童柯萨奇病毒A组9型(Coxsackie virus A9,CVA9)VP1区编码基因特征及其分子流行病学特点.方法选取1999-2002年从西藏自治区＜15岁急性弛缓性麻痹(acute flaccid paralysis,AFP)病例和≤5岁到儿童医院就诊儿童及部分健康儿童的659份粪便标本中分离的10株CVA9病毒,进行核糖核酸(RNA)提取,VP1编码区逆转录一聚合酶链反应(RT-PCR),PCR产物的核苷酸序列测定和分析.结果共分离出10株CVA9病毒,其中3株是从1999年标本中分离到的;另7株是从2000年标本分离到的,其他年份未分离到CVA9.10株CVA9病毒VP1区基因全长都是906 bp,翻译的氨基酸全长为302aa.所测10株CVA9病毒序列之间同源性为79.0%～99.9%.所测序列与CVA9原型株-Griggs株相比,同源性为75.7%～78.7%.所构建的CVA9病毒遗传进化树将已知的CVA9病毒划分为2个组,西藏自治区分离的CVA9病毒分属于1,2组.结论西藏自治区1999、2000年儿童中流行的CVA9为2个不同组,1999年流行的病毒属于2组,2000年流行的病毒属于1组,西藏自治区1999、2000年CVA9流行为不同组.2001-2002年未发现CVA9的持续流行.
목적 연구서장자치구1999-2000년≤5세인동가살기병독A조9형(Coxsackie virus A9,CVA9)VP1구편마기인특정급기분자류행병학특점.방법 선취1999-2002년종서장자치구＜15세급성이완성마비(acute flaccid paralysis,AFP)병례화≤5세도인동의원취진인동급부분건강인동적659빈분편표본중분리적10주CVA9병독,진행핵당핵산(RNA)제취,VP1편마구역전록일취합매련반응(RT-PCR),PCR산물적핵감산서렬측정화분석.결과 공분리출10주CVA9병독,기중3주시종1999년표본중분리도적;령7주시종2000년표본분리도적,기타년빈미분리도CVA9.10주CVA9병독VP1구기인전장도시906 bp,번역적안기산전장위302aa.소측10주CVA9병독서렬지간동원성위79.0%～99.9%.소측서렬여CVA9원형주-Griggs주상비,동원성위75.7%～78.7%.소구건적CVA9병독유전진화수장이지적CVA9병독화분위2개조,서장자치구분리적CVA9병독분속우1,2조.결론 서장자치구1999、2000년인동중류행적CVA9위2개불동조,1999년류행적병독속우2조,2000년류행적병독속우1조,서장자치구1999、2000년CVA9류행위불동조.2001-2002년미발현CVA9적지속류행.
Objective To study the molecular characterization of CVA9 virus in Tibet. Methods To isolate the enteroviruses from stool specimens of AFP cases and other children in Tibet in 1999-2002, and identify them by neutralization test using the RIVM antiserum; then determine the complete nucleotide sequence of VP1 region of CVA9 viruses, and analyze the results. Results A total of 10 strains of CVA9virus were isolated from the stool specimens and identified. The complete nucleotide sequence of VP1 region of these CVA9 viruses were 906nt coding 302 amino acids. To compare with the sequences of the 10 strains of Tibet, the homogeneity of nucleotide sequence were 79.0% -99.9%; while they were 75.7% -78.7%compared with Griggs. The phylogenetic tree of CVA9 viruses showed 2 groups, and the isolates from Tibet belong to 1, 2 groups. Conclusion The deduction is that the 10 strains are proposed 2 different groups, the strains epidemic in 1999 belong to group 2,while strains in 2000 belong to group 1.