CAJ | 학술논문

目的研究吉林地区腹泻患儿中人双埃柯病毒(HPeV)的流行特点和分子特征.方法采用Real-time PCR方法直接检测筛查＜5岁腹泻患儿粪便标本,应用巢氏RT-PCR扩增VP1区进行分型.结果 306例标本中,检出HPeV阳性27例(8.82%),成功分型11例,包括9例HPeV1,HPeV2和HPeV4各1例.HPeV检出集中在7月、9月和10月,7月检出率最高(28.57%).HPeV感染似乎与大于2岁患儿关系密切.VP1区基因片段与参考株核苷酸同源性为79%～92%.结论多种HPeV在我国吉林地区检出,但HPeV3未能检出,该地区HPeV的流行特征与国内外的报道基本一致,开展长期的HPeV监测对于阐明HPeV与婴幼儿腹泻及其他疾病的病因相关性有重要意义.
목적 연구길임지구복사환인중인쌍애가병독(HPeV)적류행특점화분자특정.방법 채용Real-time PCR방법 직접검측사사＜5세복사환인분편표본,응용소씨RT-PCR확증VP1구진행분형.결과 306례표본중,검출HPeV양성27례(8.82%),성공분형11례,포괄9례HPeV1,HPeV2화HPeV4각1례.HPeV검출집중재7월、9월화10월,7월검출솔최고(28.57%).HPeV감염사호여대우2세환인관계밀절.VP1구기인편단여삼고주핵감산동원성위79%～92%.결론 다충HPeV재아국길임지구검출,단HPeV3미능검출,해지구HPeV적류행특정여국내외적보도기본일치,개전장기적HPeV감측대우천명HPeV여영유인복사급기타질병적병인상관성유중요의의.
Objective To study HPeV from stool samples of children with acute gastroenteritis under 5 years old. Methods We conducted a real-time PCR to detect HPeV from stool samples and to amply VP1 sequence by nested RT-PCR to identify HPeV type. Results The results showed that 27 of 306 (8.82%) children with acute gastroenteritis were infected HPeV. 11 strains were typed. 9 strains HPeV1,both HPeV2 and HPeV4 was 1 strain. HPeV was mostly identified in autumn season with a peak in July.HPeV seemed relevant in children ＞ 2 years old. The range of nucleotide identity between all isolated strains with reference strains was 79%-92%. Conclusion Epidemiology characteristic of HPeV in Jilin was concordance with that of reports. HPeV3 wasnt detected. It's significant to conduct the large scale and longterm surveillance of HPeV.