中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2011年
1期
66-68
,共3页
李健雄%房师松%程小雯%王婷%王昕%吕星%吴春利%张仁利%程锦泉%俞慕华
李健雄%房師鬆%程小雯%王婷%王昕%呂星%吳春利%張仁利%程錦泉%俞慕華
리건웅%방사송%정소문%왕정%왕흔%려성%오춘리%장인리%정금천%유모화
流感病毒%聚合酶链反应%细胞培养%鸡胚
流感病毒%聚閤酶鏈反應%細胞培養%鷄胚
류감병독%취합매련반응%세포배양%계배
Influenza virus%Polymerase chain reaction%Cell culture%Chink embryo
目的 通过2009年下半年深圳市流感监测数据,对荧光RT-PCR,细胞培养法和鸡胚培养法进行分析比较.方法 对2009年下半年的1092份咽试子样本采用荧光RT-PCR,细胞培养法和鸡胚培养法三种方法 进行检测,对检测结果 用统计学方法 进行分析.结果 荧光RT-PCR,细胞培养法和鸡胚培养法的阳性率分别为54.21%、27.11%和16.21%,灵敏度分别为100%,50%和29.9%.荧光RT-PCR的检出限为10-2TCID50/ml.结论 荧光RT-PCR的灵敏度要明显高于培养法,而且同样具有非常高的特异度,其在流感的应急检测应用中要明显优于两种培养法.细胞培养法对H3N2亚型的敏感性高,而鸡胚培养法对B型流感的敏感性高.
目的 通過2009年下半年深圳市流感鑑測數據,對熒光RT-PCR,細胞培養法和鷄胚培養法進行分析比較.方法 對2009年下半年的1092份嚥試子樣本採用熒光RT-PCR,細胞培養法和鷄胚培養法三種方法 進行檢測,對檢測結果 用統計學方法 進行分析.結果 熒光RT-PCR,細胞培養法和鷄胚培養法的暘性率分彆為54.21%、27.11%和16.21%,靈敏度分彆為100%,50%和29.9%.熒光RT-PCR的檢齣限為10-2TCID50/ml.結論 熒光RT-PCR的靈敏度要明顯高于培養法,而且同樣具有非常高的特異度,其在流感的應急檢測應用中要明顯優于兩種培養法.細胞培養法對H3N2亞型的敏感性高,而鷄胚培養法對B型流感的敏感性高.
목적 통과2009년하반년심수시류감감측수거,대형광RT-PCR,세포배양법화계배배양법진행분석비교.방법 대2009년하반년적1092빈인시자양본채용형광RT-PCR,세포배양법화계배배양법삼충방법 진행검측,대검측결과 용통계학방법 진행분석.결과 형광RT-PCR,세포배양법화계배배양법적양성솔분별위54.21%、27.11%화16.21%,령민도분별위100%,50%화29.9%.형광RT-PCR적검출한위10-2TCID50/ml.결론 형광RT-PCR적령민도요명현고우배양법,이차동양구유비상고적특이도,기재류감적응급검측응용중요명현우우량충배양법.세포배양법대H3N2아형적민감성고,이계배배양법대B형류감적민감성고.
Objective Real-time RT-PCR, cell culture and embryonated eggs culture for influenza detection were compared by analyzing the data of influenza surveillance in Shenzhen in second half of 2009.Methods 1092 clinical samples ( throat swabs ) collected during second half of 2009 were tested by realtime RT-PCR, cell culture and embryonated eggs culture, and the results were analyzed by statistical methods. Results The positive rate were 54. 21% ,27.11% and 16. 21% using real-time RT-PCR, cell culture and embryonated eggs culture, and the sensitive were 100% ,50% and 29. 9%. The lowest dilutions of virus detected by real-time RT-PCR were 10-2TCID50/ml. Conclusion The sensitive of real-time RTPCR was higher than culture and the specificity was also very high. It was more suitable for emergency detect. The sensitive of cell culture for H3N2 subtype was higher, and sensitive of embryonated eggs culture for type B was higher.
