CAJ | 학술논문

目的了解烟台地区手足口病合并脑炎病原谱及其基因学特征.方法采集烟台地区手足口病合并脑炎患者粪便及脑脊液标本,通过细胞培养分离病毒,实时荧光PCR鉴定病毒型别,RT-PCR扩增VP1区基因部分序列并测序,进行序列分析.结果 10份粪便标本分离病毒3株,均为EV71.与C4a型代表株核苷酸同源性为98％～99％,氨基酸同源性98.90％～99.45％.系统发生树中,烟台分离株与C4亚型C4a簇代表株位于同一分支.结论烟台地区手足口病合并脑炎病原主要为EV71,属于C4亚型C4a簇.
목적 료해연태지구수족구병합병뇌염병원보급기기인학특정.방법 채집연태지구수족구병합병뇌염환자분편급뇌척액표본,통과세포배양분리병독,실시형광PCR감정병독형별,RT-PCR확증VP1구기인부분서렬병측서,진행서렬분석.결과 10빈분편표본분리병독3주,균위EV71.여C4a형대표주핵감산동원성위98％～99％,안기산동원성98.90％～99.45％.계통발생수중,연태분리주여C4아형C4a족대표주위우동일분지.결론 연태지구수족구병합병뇌염병원주요위EV71,속우C4아형C4a족.
Objective To learn about the pathogen spectrum and genetic characterization of HFMD with encephalitis in Yantai city.Methods Stool samples and cerebrospinal fluids (CSF) collected from HFMD with encephalitis cases in Yantai.Virus were isolated from stool samples and identified by fluorescence reverse transcription-polymerase chain reaction.The VP1 region was amplified and sequenced from positive specimens.Genetic characterization was identified by sequence analysis.Results Getting 3 virus strains from 10 stool specimens and all of them belong to EV71.The nucleotide and amino acid homogeneity with the representative isolates of C4a were 98％-99％ and 98.90％-99.45％ respectively.Conclusion The pathogen of HFMD with encephalitis in Yantai city were mainly EV71 wich belong to subgenogroup C4 cluster C4a.