汉坦病毒浙江分离株的基因分型
한탄병독절강분리주적기인분형
Genotype of hantavirus in Zhejiang province
  • 万方数据
    中华实验和临床病毒学杂志 중화실험화림상병독학잡지
    CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
    2012年 5期 344-347 ,共4页

    徐芳%姚苹苹%朱函坪%王洁%钱磊%胡群%童淑梅%邓小昭%谢荣辉%杨章女%张云%朱智勇

    서방%요평평%주함평%왕길%전뢰%호군%동숙매%산소소%사영휘%양장녀%장운%주지용

    汉坦病毒%基因型 漢坦病毒%基因型
    한탄병독%기인형
    Hantaan virus%Genotype
    目的 对浙江省汉坦病毒(HV)分离株进行基因分型,为制定肾综合征出血热防制对策提供科学依据.方法 应用RT-PCR的方法,使用汉坦病毒型特异性引物,对汉坦病毒浙江分离株型特异性M和S片段进行扩增和测序,并与其他已知病毒序列进行比较,以明确浙江株的基因型和亚型.结果 本研究中18株HV浙江株11株为汉滩型(HTN),分为5个亚型,另7株为汉城型(SEO),分为3个亚型.HTNV ZNB-1、ZNB-2毒株和A3毒株以及SEOV Gou3、ZJ5毒株在种系发生上与其他HTNV和SEOV明显不同,Gou3和ZJ5株在SEOV发生群中构成一独立分支,比较核苷酸序列发现,ZNB-1和ZNB-2病毒M片段除与HTNV Nc167株有较高的同源性外(88.7％),与其他HTNV的同源性在69.7％ ～74.0％之间,A3株M片段除与HTNV B78株有较高的同源性外(99.7％),与其他HTNV的同源性在73.6％ ～76.3％之间.结论 浙江省是汉坦病毒中汉滩型和汉城型的混合型疫区,HTNV至少有5个亚型,SEOV至少有3个亚型,而且浙江省存在着特殊亚型的HTN毒株以及SEO毒株.
    목적 대절강성한탄병독(HV)분리주진행기인분형,위제정신종합정출혈열방제대책제공과학의거.방법 응용RT-PCR적방법,사용한탄병독형특이성인물,대한탄병독절강분리주형특이성M화S편단진행확증화측서,병여기타이지병독서렬진행비교,이명학절강주적기인형화아형.결과 본연구중18주HV절강주11주위한탄형(HTN),분위5개아형,령7주위한성형(SEO),분위3개아형.HTNV ZNB-1、ZNB-2독주화A3독주이급SEOV Gou3、ZJ5독주재충계발생상여기타HTNV화SEOV명현불동,Gou3화ZJ5주재SEOV발생군중구성일독립분지,비교핵감산서렬발현,ZNB-1화ZNB-2병독M편단제여HTNV Nc167주유교고적동원성외(88.7％),여기타HTNV적동원성재69.7％ ～74.0％지간,A3주M편단제여HTNV B78주유교고적동원성외(99.7％),여기타HTNV적동원성재73.6％ ～76.3％지간.결론 절강성시한탄병독중한탄형화한성형적혼합형역구,HTNV지소유5개아형,SEOV지소유3개아형,이차절강성존재착특수아형적HTN독주이급SEO독주.
    Objective To identify the genotype and clades of hantavirus (HV) in Zhejiang province.Methods The partial S and M segment of the HV in Zhejiang province were amplified with RTPCR using genotype-specific primers,and then were sequenced and compared with other known hantaviruses.Results The genotype of 11 strains were HTNV and other 7 strains were SEOV by homology and phylogenesis analysis,yet the clade distribution was significantly different among foci of Zhejiang with 5 clades of HTNV and 3 clades of SEOV.There also existed special clade of HTNV named ZNB-1,ZNB-2,A3 and of SEOV named Gou3,ZJ5.The homology of M segments of ZNB-1 and ZNB-2 with other HTNV clades were 69.7％-74.0％ except Nc167,A3 with other HTNV clades were 73.6％-76.3％ except B78.Conclusion Zhejiang province is co-circulating with HTN and SEO.Say the least of the clades are 5 of HTNV and 3 of SEOV and there also existed special clade of HTNV and SEOV.
    원문보기 원문다운로드 사이트로이동
저자의 최근 논문