CAJ | 학술논문

目的分析徐州地区2005-2011年流感暴发疫情的流行特征与规律,为流感防控提供科学依据.方法采集2005-2011年流感暴发疫情病例的鼻咽拭子标本,用荧光-PCR方法检测流感病毒核酸,并快速对流感病毒A1(H1N1)、A3(H3 N2)、新H1N1、BV (Victoria)、BY (Yamagate)亚型进行分型.同时用MDCK细胞进行流感病毒分离,对所分离的流感病毒初步鉴定后,上送国家流感中心进行复核.结果 2005-2011年,徐州地区共发生流感暴发疫情53起,其中流感病毒亚型BV引起占26.42％ (14/53)、A3引起占49.1％(26/53)、A3和A1混合引起占3.77％(2/53)、新H1N1引起占20.75％(11/53).其中A3主要在2007年和2009年引起暴发,并呈现冬春季(1月)和夏秋季(9月)两个流行高峰；BV在2005-2011年间,主要集中在2-6月份引起疫情暴发.结论徐州地区2005年冬季以来,先后以流感病毒BV、A3、新H1N1等亚型交替为主要流行优势株,引起局部流感暴发疫情.其中BV在2005-2011年间,有逐年增强的趋势.中小学生是流感暴发疫情的主要人群.荧光-PCR检测方法,可作为流感暴发疫情可靠的快速诊断首选方法.
목적 분석서주지구2005-2011년류감폭발역정적류행특정여규률,위류감방공제공과학의거.방법 채집2005-2011년류감폭발역정병례적비인식자표본,용형광-PCR방법검측류감병독핵산,병쾌속대류감병독A1(H1N1)、A3(H3 N2)、신H1N1、BV (Victoria)、BY (Yamagate)아형진행분형.동시용MDCK세포진행류감병독분리,대소분리적류감병독초보감정후,상송국가류감중심진행복핵.결과 2005-2011년,서주지구공발생류감폭발역정53기,기중류감병독아형BV인기점26.42％ (14/53)、A3인기점49.1％(26/53)、A3화A1혼합인기점3.77％(2/53)、신H1N1인기점20.75％(11/53).기중A3주요재2007년화2009년인기폭발,병정현동춘계(1월)화하추계(9월)량개류행고봉；BV재2005-2011년간,주요집중재2-6월빈인기역정폭발.결론 서주지구2005년동계이래,선후이류감병독BV、A3、신H1N1등아형교체위주요류행우세주,인기국부류감폭발역정.기중BV재2005-2011년간,유축년증강적추세.중소학생시류감폭발역정적주요인군.형광-PCR검측방법,가작위류감폭발역정가고적쾌속진단수선방법.
Objective In order to provide a scientific basis for influenza prevention and control,analyzing the epidemic characteristics and laws of influenza outbreaks in Xuzhou area during 2005-2011.Method Using fluorescent-PCR method to detect influenza virus nucleic acid on Nasopharyngeal swab specimens collected from influenza outbreak cases during 2005-2011 and fast classifying influenza virus A1 (H1N1),A3 (H3N2),new H1N1 BV (Victoria) and BY (Yamagate) on subtypes.At the same time,isolating the influenza virus with MDCK cells,and sending them to the National Influenza Center for review,after the preliminary identification of the isolated influenza virus.Results During 2005-2011,there are 53 influenza outbreaks in Xuzhou area,which caused by influenza virus subtype BV accounting for 26.42％ (14/53),A3 accounting for 49.1％ (26/53),A3 and A 1 mixture accounting for 3.77％ (2/53) and the new H1N1 accounting for 20.75％ (11/53).The outbreaks in 2007 and 2009 mainly caused by A3,and show that the winter spring (January) and summer autumn (September) as two popular peaks during 2005-2011 ; BV mainly causes the outbreaks from Feb.to Jun.Conclusion In Xuzhou area,since the winter of 2005,influenza virus subtype BV,the A3,and new H1N1 has alternately as mainly predominant strain,caused local influenza outbreaks.In which BV has increased trend year by year during 2005-2011.The students in primary and secondary schools are the major crowd of influenza outbreaks.Fluorescent-PCR detection methods could be a preferred method for reliable and rapid diagnostic of epidemic influenza outbreaks.