CAJ | 학술논문

目的探讨人类白细胞抗原G(HLA-G) 14 bp插入/缺失多态性及血浆可溶性HLA-G(sHLA-G)水平与儿童肠道病毒71型(EV71)感染的相关性.方法采用聚合酶链反应结合聚丙烯酰胺凝胶电泳(PCR-PAGE)技术,对125例EV71感染重症手足口病(HFMD)患儿及133例正常对照儿童进行HLA-G 14 bp插入/缺失多态性的检测；采用酶联免疫吸附测定(ELISA)检测其中66例重型和15例危重型EV71感染HFMD患儿及133例正常对照儿童血浆中sHLA-G水平.结果 EV71感染重症HFMD组HLA-G 14 bp插入/缺失基因型多态性14 bp-/-、14 bp+/-和14 bp+/+的频率分别为49.6％、42.4％和8.0％,正常对照组分别为34.6％、48.9％和16.5％,两组基因型多态性分布频率差异有统计学意义(x2=7.850,P=0.020)；两组等位基因分布频率差异有统计学意义(x2=7.830,P=0.005,EV71感染重型组血浆sHLA-G水平明显高于正常对照组(Z=-9.692,P=0.000),危重型组血浆中sHLA-G水平明显高于重型组(Z=-2.420,P=0.016).结论 HLA-G 14 bp插入/缺失基因型多态性与儿童EV71感染有关联,血浆sHLA-G水平可作为EV71感染的辅助诊断指标.
목적 탐토인류백세포항원G(HLA-G) 14 bp삽입/결실다태성급혈장가용성HLA-G(sHLA-G)수평여인동장도병독71형(EV71)감염적상관성.방법 채용취합매련반응결합취병희선알응효전영(PCR-PAGE)기술,대125례EV71감염중증수족구병(HFMD)환인급133례정상대조인동진행HLA-G 14 bp삽입/결실다태성적검측；채용매련면역흡부측정(ELISA)검측기중66례중형화15례위중형EV71감염HFMD환인급133례정상대조인동혈장중sHLA-G수평.결과 EV71감염중증HFMD조HLA-G 14 bp삽입/결실기인형다태성14 bp-/-、14 bp+/-화14 bp+/+적빈솔분별위49.6％、42.4％화8.0％,정상대조조분별위34.6％、48.9％화16.5％,량조기인형다태성분포빈솔차이유통계학의의(x2=7.850,P=0.020)；량조등위기인분포빈솔차이유통계학의의(x2=7.830,P=0.005,EV71감염중형조혈장sHLA-G수평명현고우정상대조조(Z=-9.692,P=0.000),위중형조혈장중sHLA-G수평명현고우중형조(Z=-2.420,P=0.016).결론 HLA-G 14 bp삽입/결실기인형다태성여인동EV71감염유관련,혈장sHLA-G수평가작위EV71감염적보조진단지표.
Objective Explore the relationship between the HLA-G 14bp insertion/deletion polymorphism and the infection of Enterovirus 71 (EV71) for children.Methods We genotyped HLA-G 14bp insertion/deletion polymorphism of 125 severe HFMD children infected with EV71 and 133 normal controls by PCR-PAGE;detected the plasma sHLA-G level of 66 heavy type and 15 critical type and 133 normal controls by ELISA.Results Frequencies of the genotype 14 bp-/-,14 bp + /-and 14 bp + / + were 49.6％,42.4％ and 8.0％ for the severe HFMD children infected with EV71,and 34.6％,48.9％ and 16.5％ for the normal controls,respectively.A significant difference was observed for the frequencies of the HLA-G 14bp genotype between the two groups(x2 =7.850,P =0.020).And for the allele frequencies.The plasma sHLA-G levels in heavy type were dramatically higher than that in normal controls(Z =-9.692,P =0.000).The plasma sHLA-G levels in children with critical HFMD were dramatically higher than that with heavy type(Z=-2.420,P =0.016).Conclusion There was a relationship between the HLA-G 14 bp insertion/deletion polymorphism and the susceptibility to the severe HFMD children infected with EV71 and the plasma sHLA-G might be considered as a index for auxiliary diagnosis the severe HFMD infected with EV71.