CAJ | 학술논문

目的观察唑来膦酸和γδ TCR单抗两种诱导方法对HIV感染者的Vδ2 T细胞的扩增效果,探讨效率更高的扩增方法.方法选择65例HIV感染者和38例健康对照者,应用唑来膦酸法和γδ TCR单抗法分别诱导其外周血单个核细胞(PBMC),分别在培养的0d、7d、14 d应用流式细胞术检测其γδ T细胞及其亚群的百分率及Vδ2 T细胞增殖情况,比较两种方法的诱导效果.结果外周血中HIV感染者Vδ2 T细胞的数量和比例与健康对照相比显著下降；扩增14 d后,γδ TCR单抗法诱导HIV感染者和健康对照者的Vδ2 T细胞占CD3+细胞的百分比分别为(17.6±19.8)％和(64.3±4.5)％,Vδ2 T细胞扩增倍数分别为54±40和74±29；而采用唑来膦酸法的诱导结果分别为(69.6±21.2)％和(97.3±1.7)％以及538±11和5984±721.结论唑来膦酸法可诱导HIV感染者及健康对照者Vδ2 T细胞大量增殖,其效率显著高于γδ TCR单抗诱导法.
목적 관찰서래련산화γδ TCR단항량충유도방법대HIV감염자적Vδ2 T세포적확증효과,탐토효솔경고적확증방법.방법 선택65례HIV감염자화38례건강대조자,응용서래련산법화γδ TCR단항법분별유도기외주혈단개핵세포(PBMC),분별재배양적0d、7d、14 d응용류식세포술검측기γδ T세포급기아군적백분솔급Vδ2 T세포증식정황,비교량충방법적유도효과.결과 외주혈중HIV감염자Vδ2 T세포적수량화비례여건강대조상비현저하강；확증14 d후,γδ TCR단항법유도HIV감염자화건강대조자적Vδ2 T세포점CD3+세포적백분비분별위(17.6±19.8)％화(64.3±4.5)％,Vδ2 T세포확증배수분별위54±40화74±29；이채용서래련산법적유도결과분별위(69.6±21.2)％화(97.3±1.7)％이급538±11화5984±721.결론 서래련산법가유도HIV감염자급건강대조자Vδ2 T세포대량증식,기효솔현저고우γδ TCR단항유도법.
Objective To find out the more efficient induction method through investigating the expansion efficiencies of HIV-infected patients' Vdelta2 T cells induced by zoledronic acid (Zol) or gammadelta TCR monoclonal antibody (mAb).Methods 38 healthy control subjects (HC group) and 65 HIV infected patients (HIV group) were enrolled in this research.Peripheral blood mononuclear cells (PBMCs) of individuals were stimulated by Zol or γδ TCR mAb respectively for 14 days at 2.0 × 107 cells/well,and then γδ T cells and Vδ2 subsets frequencies were measured by flow cytometry (FCM) on 0,7 and 14 day.The absolute numbers of Vδ2 T cells were calculated and the Vδ2 T cell expansion efficiencies by these two methods were compared.Results The absolute numbers and frequencies of Vδ2 T cell of HIV groups were lower than those of HC groups significantly on 0 day.After 14 days,the frequencies of Vδ2 T cell of HIV group and HC group were(17.6 ± 19.8)％ and(64.3 ± 4.5) ％ respectively,and the expansion indexes of Vδ2 T cell were 54 ± 40 and 74 ± 29 respectively by induction of γδ TCR mAb.However,the frequencies of Vδ2 T cell of HIV group and HC group were(69.6 ± 21.2)％ and(97.3 ± 1.7)％ respectively,and the Vδ2 T cell expansion indexes were 538 ± 11 and 5984 ±721 respectively by induction of Zol.Conclusion Zol could induce the vast expansion of Vδ2 T cells of HIV infected patients.The expansion efficiency by Zol was better than that by the γδ TCR mAb.