中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2012年
6期
453-455
,共3页
焦秀娟%彭勋%焦秀敏%王晋生%孙谢文%赵培丽%王守云%刘加群%李彤%杨静娴
焦秀娟%彭勛%焦秀敏%王晉生%孫謝文%趙培麗%王守雲%劉加群%李彤%楊靜嫻
초수연%팽훈%초수민%왕진생%손사문%조배려%왕수운%류가군%리동%양정한
肝炎病毒,乙型%基因型%药物耐受性%苷类
肝炎病毒,乙型%基因型%藥物耐受性%苷類
간염병독,을형%기인형%약물내수성%감류
Hepatitis B virus%Genotype%Tolerance%Nucleosides
目的 分析使用过核苷(酸)类似物(NA)治疗的慢性乙型肝炎患者体内HBV RT区基因耐药特点.方法 收集229例接受过核苷类似物治疗的慢性乙型肝炎患者的血清,应用snPCR法扩增HBV全长RT区,纯化snPCR产物直接测序后分析11个经典耐药位点的氨基酸变异情况.结果 229例患者中基因B和C型各占14.41％ (33/229)和85.59％ (196/229).且C型患者较B型更易发生耐药变异(x2=2.95,P＜0.05).共有63例检出HBV耐药变异,在rtI169、rtT184、rtA194和rtS202位点上均未检出耐药变异.在63个耐药变异株中rtM204V/I变异株检出率最高(40/63,63.49％),共有11种耐药突变模式.其中rtM204I以与rtL80I/V或rtL180M联合变异为主,而rtM204V变异常伴有rtL180M变异.结论 使用过核苷(酸)类似物(NA)治疗的慢性乙型肝炎患者体内HBV RT区基因耐药呈现出复杂的变异类型,rtM204V/I突变最为常见.
目的 分析使用過覈苷(痠)類似物(NA)治療的慢性乙型肝炎患者體內HBV RT區基因耐藥特點.方法 收集229例接受過覈苷類似物治療的慢性乙型肝炎患者的血清,應用snPCR法擴增HBV全長RT區,純化snPCR產物直接測序後分析11箇經典耐藥位點的氨基痠變異情況.結果 229例患者中基因B和C型各佔14.41％ (33/229)和85.59％ (196/229).且C型患者較B型更易髮生耐藥變異(x2=2.95,P＜0.05).共有63例檢齣HBV耐藥變異,在rtI169、rtT184、rtA194和rtS202位點上均未檢齣耐藥變異.在63箇耐藥變異株中rtM204V/I變異株檢齣率最高(40/63,63.49％),共有11種耐藥突變模式.其中rtM204I以與rtL80I/V或rtL180M聯閤變異為主,而rtM204V變異常伴有rtL180M變異.結論 使用過覈苷(痠)類似物(NA)治療的慢性乙型肝炎患者體內HBV RT區基因耐藥呈現齣複雜的變異類型,rtM204V/I突變最為常見.
목적 분석사용과핵감(산)유사물(NA)치료적만성을형간염환자체내HBV RT구기인내약특점.방법 수집229례접수과핵감유사물치료적만성을형간염환자적혈청,응용snPCR법확증HBV전장RT구,순화snPCR산물직접측서후분석11개경전내약위점적안기산변이정황.결과 229례환자중기인B화C형각점14.41％ (33/229)화85.59％ (196/229).차C형환자교B형경역발생내약변이(x2=2.95,P＜0.05).공유63례검출HBV내약변이,재rtI169、rtT184、rtA194화rtS202위점상균미검출내약변이.재63개내약변이주중rtM204V/I변이주검출솔최고(40/63,63.49％),공유11충내약돌변모식.기중rtM204I이여rtL80I/V혹rtL180M연합변이위주,이rtM204V변이상반유rtL180M변이.결론 사용과핵감(산)유사물(NA)치료적만성을형간염환자체내HBV RT구기인내약정현출복잡적변이류형,rtM204V/I돌변최위상견.
Objective To characterize genotypic resistance within HBV RT region in chronic hepatitis B (CHB) patients with nucleos(t) ide analogue (NA) treatment.Methods Serum samples of 229 CHB patients with NA treatment were obtained.Full-length HBV RT sequences were amplified,sequenced and analyzed,on the following NA resistant (NAr) mutations belonging to different NAr pathways.Results Among 229 HBV isolates,14.41％ (33/229) and 85.59％ (196/229) were genotype B and C,respectively;and the patients with HBV genotype C may be more susceptible to develope resistant mutations than patients with HBV genotype B (x2 =2.95,P ＜ 0.05).NAr mutations were detected in 63 CHB patients.Mutations were not found at rtI169,rtT184,rtA194 or rtS202.RtM204 mutations were detected at the highest frequency among 63 mutants (40/63,63.49％) and found to display 11 combination mutation patterns,in which rtM204I were associated with rtL80I/V and rtL180M,and rtM204V were associated with rtL180M,respectively.Conclusions There are complicated mutation patterns in the HBV RT region for chronic hepatitis B (CHB) patients with nucleos(t) ide analogue (NA) treatment.RtM204V/I mutation was the highest.
