CAJ | 학술논문

目的建立一种更加快速简便的环介导恒温扩增技术(LAMP)检测甲型肝炎病毒(HAV).方法本研究通过在传统LAMP的基础上加入加速引物建立了一种改进型的LAMP体系.结果精确性和可重复性实验证实改进后的HAV LAMP检测体系稳定可靠,重复性强,并且较传统的LAMP灵敏度更高,检测极限可以达到5 TCID50/ml.当该方法用于甲型肝炎患者急性期血清临床样本的检测时,实验结果与TaqMan实时荧光定量PCR的检测结果一致.结论改进型的LAMP技术有望应用于HAV临床样本检测,同时也适用于HAV流行地区的病原学监测和调查.
목적 건립일충경가쾌속간편적배개도항온확증기술(LAMP)검측갑형간염병독(HAV).방법 본연구통과재전통LAMP적기출상가입가속인물건립료일충개진형적LAMP체계.결과 정학성화가중복성실험증실개진후적HAV LAMP검측체계은정가고,중복성강,병차교전통적LAMP령민도경고,검측겁한가이체도5 TCID50/ml.당해방법용우갑형간염환자급성기혈청림상양본적검측시,실험결과여TaqMan실시형광정량PCR적검측결과일치.결론 개진형적LAMP기술유망응용우HAV림상양본검측,동시야괄용우HAV류행지구적병원학감측화조사.
Objective To establish a novel improved loop-mediated isothermal amplification (LAMP) technique to detect hepatitis A virus (HAV).Methods A novel improved LAMP assay based on the addition of an acceleration primer was developed for hepatitis A virus nucleotide acid detection.Results Precision and reproducibility analysis proved its high stability and reliability.Comparison between the improved and conventional LAMP assays revealed that the former was more sensitive with a detection limit of 5 TCID5o/ml.The novel detection method displayed 100％ consistency with the TaqMan real-time PCR assay when applied to clinical specimens collected from patients with confirmed acute HAV infection.Conclusion This novel technique is widely applicable as a simple diagnostic tool in the clinical field as well as for the surveillance and investigation of the infectious disease in developing countries where HAV is endemic.