CAJ | 학술논문

目的分析陕西省发热呼吸道症候群患者咽拭子中16种呼吸道病毒病原谱构成和呼吸道合胞病毒基因特征.方法 2010年1月至2011年1月收集符合监测定义患者咽拭子标本208份,采用基于毛细管电泳的多重RT-PCR反应进行病毒核酸检测,包括人鼻病毒(HRV)、冠状病毒(HCOV)、流感病毒(Flu)、副流感病毒(HPIV)、腺病毒(ADV)、呼吸道合胞病毒(HRSV)、人偏肺病毒(HMPV)以及博卡病毒(HBOV),并对其中HRSV检测阳性的标本进行G基因羧基末端测序与种系进化分析,研究流行于陕西的HRSV分子流行病学特征.结果总病毒检出率为53％(109/208),检测到的阳性病毒病原构成率分别为:HRSV42.2％、Flu24.5％、PIV20％,HRV13.6％,ADV10.9％,HCOV7.3％、HMPV4.6％和3份HBOV2.7％.有20％(22/109)病例为混合病毒感染,其中有14例为PIV与其他病毒混合感染,15 ～ 39岁年龄组混合感染率最高,差异有统计学意义(P＜0.05).总呼吸道病毒的感染率在各年龄组中差别不大,除Flu、HMPV和PIV外,其他病毒(HRV、HBoV、HCoV、ADV、HRSV)的感染人群主要以0～4岁小儿为主.46例多重RT-PCR HRSV阳性病例中,42例为HRSV A血清型的NA1基因型；2例为B血清型,其中1例为BA9基因型,1例为GB2基因型.结论 @@陕西发热呼吸道症候群由多种呼吸道病毒引起,存在2种或以上病毒的混合感染,其中以PIV与其他病毒混合感染为主.HRSV是2010年陕西省发热呼吸道症候群的主要病原,其中A血清型的NA1基因型是陕西2010年流行的绝对优势型别.
목적 분석합서성발열호흡도증후군환자인식자중16충호흡도병독병원보구성화호흡도합포병독기인특정.방법 2010년1월지2011년1월수집부합감측정의환자인식자표본208빈,채용기우모세관전영적다중RT-PCR반응진행병독핵산검측,포괄인비병독(HRV)、관상병독(HCOV)、류감병독(Flu)、부류감병독(HPIV)、선병독(ADV)、호흡도합포병독(HRSV)、인편폐병독(HMPV)이급박잡병독(HBOV),병대기중HRSV검측양성적표본진행G기인최기말단측서여충계진화분석,연구류행우협서적HRSV분자류행병학특정.결과 총병독검출솔위53％(109/208),검측도적양성병독병원구성솔분별위:HRSV42.2％、Flu24.5％、PIV20％,HRV13.6％,ADV10.9％,HCOV7.3％、HMPV4.6％화3빈HBOV2.7％.유20％(22/109)병례위혼합병독감염,기중유14례위PIV여기타병독혼합감염,15 ～ 39세년령조혼합감염솔최고,차이유통계학의의(P＜0.05).총호흡도병독적감염솔재각년령조중차별불대,제Flu、HMPV화PIV외,기타병독(HRV、HBoV、HCoV、ADV、HRSV)적감염인군주요이0～4세소인위주.46례다중RT-PCR HRSV양성병례중,42례위HRSV A혈청형적NA1기인형；2례위B혈청형,기중1례위BA9기인형,1례위GB2기인형.결론 @@협서발열호흡도증후군유다충호흡도병독인기,존재2충혹이상병독적혼합감염,기중이PIV여기타병독혼합감염위주.HRSV시2010년합서성발열호흡도증후군적주요병원,기중A혈청형적NA1기인형시협서2010년류행적절대우세형별.
Objective Analysis the viral pathogenic spectrum for patients with fever and respiratory tract infection syndrome in Shaanxi province during 2010 and investigate the molecular epidemiology characteristics of respiratory syncytial virus.Methods A total of 208 patients' pharyngeal swabs were collected based on surveillance definition from January 2010 to January 2011 and screened for sixteen human respiratory virus types/subtypes by Qiaxcel-based multiplex reverse transcription-PCR assay,including HRV,HCoV,Flu,HPIV,ADV,HRSV,HMPV and HBoV and investigate molecular epidemiology of HRSV by sequencing and phylogenetic analysis of the C-terminal second hypervariable region of the G gene.Results 109 out of 208 specimens (53％) were positive for one or more viruses.HRSV(42.2％) was the dominant pathogen detected,followed by Flu(24.5％),PIV(20％),HRV(13.6％) and ADV(10.9％),there were also 8 strains of HCoV,5 strains of HMPV and 3 strains of HBoV detected.The results showed that 22specimens were positive for two or more viruses,PIV (14/22) was the most frequently detected viral agent among co-infection specimens,and the highest incidence of mixed infection is aged 15-39 years group (P ＜0.05).The overall viral detection rate was no related to age.In addition to Flu,HMPV and PIV,other viruses (HRV,HBoV,HCoV,ADV,RSV) mainly infected 0 to 4 years old children.Among 46 HRSV positive specimens,42 HRSV-A strains clustered into NAI genotype and two HRSV-B strains clustered into two genotypes,BA9 and GB2.Conclusion HRSV is the dominate pathogen collected from patients with fever and respiratory tract infection syndrome in Shaanxi and HRSV A is the predominant subtype.For most viruses,infection was most prevalent among children aged ＜ 4 years.PIV was the most common pathogen in co-infection.