CAJ | 학술논문

目的了解喉鳞状细胞癌患者人乳头瘤病毒感染的情况.方法对收集到的64例临床诊断喉癌病例的石蜡组织标本应用Luminex及PCR的方法对HPV感染进行基因检测,应用免疫组织化学的方法对HPV16/18E6蛋白进行检测.结果在64例临床诊断的喉癌病例中,通过Luminex及PCR的方法发现7例病例具有HPV的感染；通过免疫组织化学的方法发现18例病例具有HPV16/18的感染,基因及蛋白检测的总阳性率达到39.1％.结论此研究发现在喉癌患者中较高的HPV感染率间接说明HPV感染对喉癌发生的重要性,为阐明喉癌的发病机制奠定基础.
목적 료해후린상세포암환자인유두류병독감염적정황.방법 대수집도적64례림상진단후암병례적석사조직표본응용Luminex급PCR적방법대HPV감염진행기인검측,응용면역조직화학적방법대HPV16/18E6단백진행검측.결과 재64례림상진단적후암병례중,통과Luminex급PCR적방법발현7례병례구유HPV적감염；통과면역조직화학적방법발현18례병례구유HPV16/18적감염,기인급단백검측적총양성솔체도39.1％.결론 차연구발현재후암환자중교고적HPV감염솔간접설명HPV감염대후암발생적중요성,위천명후암적발병궤제전정기출.
Objective To analyze the infection of human papillomavirus in laryngeal squamous cell carcinoma patients.Methods The pathological samples of 64 clinical diagnosed laryngeal squamous cell carcinoma patients were collected.Lunimex and PCR techniques were used to detect the HPV gene infection and immunohistochemistry method was used to analyze the HPV protein expression in the samples.Results In the 64 cases,7 were positive for HPV infection by Luminex and PCR tests.18 were positive for HPV16/18 E6 protein expression.The total positive rate was about 39.1％.Conclusion The high HPV infection rate in laryngeal squamous cell carcinoma patients in the study indicated indirectly that the importance of the HPV infection in pathogenesis of the laryngeal squamous cell carcinoma.