CAJ | 학술논문

目的了解湖南地区流行性腮腺炎(流腮)病毒流行株基因特性及变异情况.方法采用Vero/SLAM细胞分离流行性腮腺炎病毒,通过小疏水蛋白基因(SH基因)的序列分析,与其他基因型参考株进行同源性比较,构建亲缘进化树.结果从2011年湖南多地区上送的16份咽拭子标本中分离到腮腺炎病毒4株,通过基因特性分析,4株毒株均属于F基因型.结论 2011年湖南省流行性腮腺炎的流行株为F基因型,未发现基因型变异.
목적 료해호남지구류행성시선염(류시)병독류행주기인특성급변이정황.방법 채용Vero/SLAM세포분리류행성시선염병독,통과소소수단백기인(SH기인)적서렬분석,여기타기인형삼고주진행동원성비교,구건친연진화수.결과 종2011년호남다지구상송적16빈인식자표본중분리도시선염병독4주,통과기인특성분석,4주독주균속우F기인형.결론 2011년호남성류행성시선염적류행주위F기인형,미발현기인형변이.
Objective To be acquainted with genetic characteristics and variation of mumps virus strains circulating in Hunan province.Methods Mumps virus (MV) strains were isolated using Vero/SLAM cells.The small hydrophobic protein (SH) genes of MV isolates were sequenced,and the sequences were analysed phylogenetically between the isolated strains and other reference mumps strains.Results 4 mumps virus strains were isolated from 16 specimens collected in 2011 from different regions of Hunan province.The genotype of isolated strains were supposed to be F type.Conclusion Genotype F is the main genotype of circulating strains in Hunan province in 2011 and there is no variation between genotype.