CAJ | 학술논문

目的研究铝佐剂和免疫程序对不同配比的Sabin IPV免疫原性的影响.方法用Ⅰ、Ⅱ、Ⅲ型脊髓灰质炎病毒原液制备四批Sabin IPV,分别为中剂量无佐剂组、中剂量铝佐剂组、低剂量无佐剂组、低剂量铝佐剂组,采用0、1、2月和0、2、4月两种免疫程序对大鼠进行3针基础免疫,每针基础免疫一个月后采血,并检测免疫后血清中3个型别的中和抗体水平.结果经过3针基础免疫后,各组Ⅰ、Ⅱ、Ⅲ中和抗体几何平均滴度均显著提高,阳转率均达到100％.对不同组别间两种免疫程序比较无显著差异,但相同条件下,0、2、4月免疫程序可诱导产生更高的抗体水平.对相同免疫程序下不同组别进行比对,添加铝佐剂组与无佐剂组相比可以诱导更高的抗体水平.结论铝佐剂和免疫程序可提高Sabin IPV免疫原性.
목적 연구려좌제화면역정서대불동배비적Sabin IPV면역원성적영향.방법 용Ⅰ、Ⅱ、Ⅲ형척수회질염병독원액제비사비Sabin IPV,분별위중제량무좌제조、중제량려좌제조、저제량무좌제조、저제량려좌제조,채용0、1、2월화0、2、4월량충면역정서대대서진행3침기출면역,매침기출면역일개월후채혈,병검측면역후혈청중3개형별적중화항체수평.결과 경과3침기출면역후,각조Ⅰ、Ⅱ、Ⅲ중화항체궤하평균적도균현저제고,양전솔균체도100％.대불동조별간량충면역정서비교무현저차이,단상동조건하,0、2、4월면역정서가유도산생경고적항체수평.대상동면역정서하불동조별진행비대,첨가려좌제조여무좌제조상비가이유도경고적항체수평.결론 려좌제화면역정서가제고Sabin IPV면역원성.
Objective To study the effect of aluminume adjuvant and immunization schedule on immunogenicity of Sabin inactivated poliovirus vaccine.Methods Four batches of Sabin IPV were produced by different concentrations of type1,2,and 3 poliovirus and administrated on three-dose schedule at 0,1,2 months and 0,2,4 months on rats.Serum samples were collected one month after each dose and neutralizing antibody titers against three types poliovirus were determined by micro-neutralization assay.Results The GMTs of neutralizing antibodies against three types poliovirus increased significantly and the seropositivity rates were 100％ in all groups after 3 doses.There was no significant difference between two immunization schedules,and the 0,2,4 month schedule could induce higher level neutralizing antibody compared to the 0,1,2 month schedule.The groups with aluminum adjuvant could induce higher level neutralizing antibody compared to the groups without adjuvant.Conclusion Aluminum djuvant and immunization schedule could improve the immunogenicity of Sabin IPV.