CAJ | 학술논문

目的建立基于核蛋白双抗体夹心ELISA滴定发热伴血小板减少综合征病毒(SFTSV)的方法.方法首先利用SFTSV核蛋白特异性多克隆和单克隆抗体建立双抗体夹心ELISA方法,用于SFTSV病毒滴度的检测,优化检测程序,评价检测方法的特异性与灵敏性,并与免疫荧光法和空斑试验法检测SFTSV滴度的方法进行比较.结果所建立的基于双抗体夹心ELISA法滴定病毒与免疫荧光法和空斑试验法的滴定结果一致,相关系数分别为0.999和0.949.结论基于SFTSV核蛋白双抗体夹心ELISA的滴定方法具有较高敏感性和特异性,操作简单,避免直接免疫荧光和空斑法滴定结果判定的主观性,并可适用于高通量检测.
목적 건립기우핵단백쌍항체협심ELISA적정발열반혈소판감소종합정병독(SFTSV)적방법.방법 수선이용SFTSV핵단백특이성다극륭화단극륭항체건립쌍항체협심ELISA방법,용우SFTSV병독적도적검측,우화검측정서,평개검측방법적특이성여령민성,병여면역형광법화공반시험법검측SFTSV적도적방법진행비교.결과 소건립적기우쌍항체협심ELISA법적정병독여면역형광법화공반시험법적적정결과일치,상관계수분별위0.999화0.949.결론 기우SFTSV핵단백쌍항체협심ELISA적적정방법구유교고민감성화특이성,조작간단,피면직접면역형광화공반법적정결과판정적주관성,병가괄용우고통량검측.
Objective To develop an assay for titration of severe fever with thrombocytopenia syndrome virus (SFTSV) based on double antibody sandwich ELISA.Methods A double antibody sandwich ELISA was developed for detection of SFTSV based on SFTSV nucleocapsid (N) protein specific poly-and monoclonal antibodies,procedures were optimized and evaluated.This ELISA based titration assay was compared with fluorescence assasy and plaque assay based titration method.Results The results suggested that the titers obtained by ELISA based method are consistent with those obtained by IFA based method(R =0.999) and the plaque assay titration method (R =0.949).Conclusion The novel ELISA based titration method with high sensitivity and specificity is easy to manage and perform,and can overcome the subjectivity associated with result determination of the fluorescence assay and plaque assay based methods.The novel ELISA based titration method can also be applied to high throughput detection.